Dewes E
Arbeitsgruppe Prof. Mosbacher, Fachbereich Biologie-Zoologie-der Universität des Saarlandes, D-6600, Saarbrücken 11, Bundesrepublik Deutschalnd.
Wilehm Roux Arch Dev Biol. 1975 Jun;178(2):167-183. doi: 10.1007/BF00848395.
Complete and bisected male genital disks (HO) from larvae of the last larval instar were transplanted into full-grown (T7) larvae. In experiment A the HO were taken from T1-, T3-, T5- and T7-larvae and either implanted intact into the host larva (group A/a) or both halves of the bisected disk were transferred into a single host (group A/b). In experiment B the HO from T1-, T4- and T7-larvae were bisected and both halves were transplanted either into a single host (group B/b) or into two different hosts (group B/c).After metamorphosis of the hosts the "complete" transplants (A/a) of all age groups showed the same morphological features: qualitative differences were not observed. Within each experimental group the size of the genitalia varied considerably. However, it was found that the variation amongst the average sized genitalia of all groups was not great.After transplantation of both halves of the bisected HO (A/b, B/b) three classes of results were observed: I. each of the two halves regenerated into a complete genital organ (2r); II. only one of the halves regenerated into a complete organ (1r); III. none of the two halves regenerated the missing part (Or). Frequency of Regeneration. A/b: The frequency of regeneration (ratio 2r: 1r: Or) was the same in the age groups T1 and T3 and in T5 and T7, but the latter, older transplants regenerated more frequently than the former. B/c: In the three age groups only on part of the transplanted halves regenerated. The ratio of the regenerated halves to the non-regenerated genital organs in the three age groups did not differ significantly. Comparing the results of the groups B/c and B/b, the frequency of regeneration was found to increase by implantation of two halves in a single host. Size of the Regenerated Genital Organs. After metamorphosis of the hosts the regenerated genitalia of all groups differentiated in much the same way as "complete" transplants, but they were slightly smaller than the latter. Comparison of the size of the genitalia in exp. B showed that implantation of two halves in a single host did not influence the size of the regenerating genitalia. Duration of Development of the Hosts. The duration of further development of the hosts was neither influenced by the transplantation of "complete" disks of different age nor when the implanted halves of the bisected HO did not regenerate. On the contrary, the regeneration of implanted HO-halves caused a delay of further development in the hosts (i.e. the onset of pupation was delayed). Independently of the size and developmental stage of the disks at the time of transplantation, when only one half regenerated the average delay was almost two days as compared to the controls. An additional delay was recorded when both implanted halves had regenerated.The developmental capacities of the transplanted disks and the control of metamorphosis by regenerating disks are discussed.
将末龄幼虫的完整和二分的雄性生殖盘（HO）移植到成熟（T7）幼虫中。在实验A中，HO取自T1、T3、T5和T7幼虫，要么完整植入宿主幼虫（A/a组），要么将二分盘的两半转移到单个宿主中（A/b组）。在实验B中，将来自T1、T4和T7幼虫的HO二分，两半分别移植到单个宿主中（B/b组）或两个不同宿主中（B/c组）。宿主变态后，所有年龄组的“完整”移植（A/a）均显示相同的形态特征：未观察到质的差异。每个实验组内生殖器的大小差异很大。然而，发现所有组平均大小生殖器之间的差异不大。将二分HO的两半移植后（A/b、B/b），观察到三类结果：I.两半中的每一半都再生为一个完整的生殖器官（2r）；II.只有一半再生为一个完整的器官（1r）；III.两半均未再生缺失部分（0r）。再生频率。A/b：T1和T3年龄组以及T5和T7年龄组的再生频率（2r:1r:0r比例）相同，但后者，即年龄较大的移植体再生频率高于前者。B/c：在三个年龄组中，只有部分移植的半体再生。三个年龄组中再生半体与未再生生殖器官的比例无显著差异。比较B/c组和B/b组的结果，发现将两半植入单个宿主中可提高再生频率。再生生殖器官的大小。宿主变态后，所有组再生的生殖器与“完整”移植的分化方式大致相同，但略小于后者。实验B中生殖器大小的比较表明，将两半植入单个宿主中不影响再生生殖器的大小。宿主发育持续时间。宿主进一步发育的持续时间既不受不同年龄“完整”盘移植的影响，也不受二分HO植入半体未再生的影响。相反，植入HO半体的再生会导致宿主进一步发育延迟（即化蛹开始延迟）。与对照组相比，无论移植时盘的大小和发育阶段如何，当只有一半再生时，平均延迟近两天。当植入的两半都再生时，记录到额外的延迟。讨论了移植盘的发育能力以及再生盘对变态的控制。
