Asashima Makoto
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000, Berlin 33, Germany.
Wilehm Roux Arch Dev Biol. 1980 Jun;188(2):123-126. doi: 10.1007/BF00848803.
Parts of the grey crescent region of 1-2 cell, 8 cell and morula stages ofAmbystoma mexicanum were combined with presumptive gastrula ectoderm of the same species (sandwich method).Grey crescent material of the early cleavage stages induced neural tissues at a very low rate (6%-7%).From the morula stage onwards, the inducing ability of the grey crescent area increased and led to the formation of mesodermal as well as neural organs. The implanted area did not participate in the organ formations.
将美西螈1 - 2细胞期、8细胞期和桑椹胚期的灰色新月区部分与同一物种的假定原肠胚外胚层相结合（三明治法）。早期卵裂阶段的灰色新月物质诱导神经组织的发生率非常低（6% - 7%）。从桑椹胚期开始，灰色新月区的诱导能力增强，并导致中胚层以及神经器官的形成。植入区域未参与器官形成。
