Lheureux Emile
Laboratoire de Biologie Animale, Université des Sciences et Techniques de Lille, France.
Wilhelm Roux Arch Entwickl Mech Org. 1975 Dec;176(4):303-327. doi: 10.1007/BF00575323.
X-irradiation suppresses the morphogenetic power of limb tissues. Transplantation of non-irradiated tissues onto an irradiated limb allows, one to study the influence of the grafts qualities (viz. dorsal, ventral, anterior and posterior qualities) and the influence of the orientation of these grafts both on the degree of development and on the orientation of the regenerates. Either limb tissues or flank tissues were grafted. Either four or two or one quality were represented in the limb tissue grafts, while various orientations and locations were imposed upon them. 1. When four qualities (dorsal, ventral, anterior and posterior) were present in the grafts, regeneration was generally normal and complete. When two qualities (dorsal and ventral) were present the rate of normal or subnormal regeneration was 66%, with differentiation of all skeletal parts of the regnerarates. When only one quality (dorsal or ventral) was present regenerates were deficient or absent, and differentiation of carpus and zeugopod bones was never observed. At least two opposite qualities are necessary to allow regenerates to develop. 2. When we graft skin cuffs on irradiated limbs, the proximo-distal development of the regenerates depends only upon the proximo-distal level of the non-irradiated limb from which the graft was taken. 3. The orientation of regenerates is like that of the grafts. 4. 90‡ rotation of the skin has no influence on regeneration.
X射线照射会抑制肢体组织的形态发生能力。将未受照射的组织移植到受照射的肢体上，人们就可以研究移植物特性（即背侧、腹侧、前侧和后侧特性）的影响，以及这些移植物的方向对再生程度和再生体方向的影响。移植的组织既有肢体组织，也有胁腹组织。肢体组织移植物中呈现出四种、两种或一种特性，同时赋予它们不同的方向和位置。1. 当移植物中存在四种特性（背侧、腹侧、前侧和后侧）时，再生通常是正常且完全的。当存在两种特性（背侧和腹侧）时，正常或亚正常再生的比例为66%，再生体的所有骨骼部分都会分化。当只存在一种特性（背侧或腹侧）时，再生体发育不全或不存在，腕骨和胫跗骨的分化从未观察到。至少需要两种相对的特性才能使再生体发育。2. 当我们将皮肤袖口移植到受照射的肢体上时，再生体的近远侧发育仅取决于取自未受照射肢体的近远侧水平。3. 再生体的方向与移植物的方向相同。4. 皮肤旋转90°对再生没有影响。
