Matthews P A, Baldo B A, Howden M E
School of Chemistry, Macquarie University, Sydney, Australia.
Mol Immunol. 1988 Jan;25(1):63-8. doi: 10.1016/0161-5890(88)90091-0.
Two cytochrome c allergens were isolated from extracts of the pollens of the dicotyledons English plantain (Plantago lanceolata) and Paterson's Curse (Echium plantagineum) by ion exchange chromatography, gel filtration and preparative isoelectric focusing. They were characterized by their absorption spectra, mol. wt, pI and amino acid composition. The cytochromes c bound specific IgE in the sera of hypersensitive patients by RAST. Preliminary evidence for allergenic cross-reactivity between them was obtained by RAST inhibition.
通过离子交换色谱法、凝胶过滤法和制备性等电聚焦法,从双子叶植物英国车前草(Plantago lanceolata)和刺苞菜蓟(Echium plantagineum)的花粉提取物中分离出两种细胞色素c过敏原。通过吸收光谱、分子量、等电点和氨基酸组成对它们进行了表征。通过放射性变应原吸附试验(RAST),细胞色素c与过敏患者血清中的特异性IgE结合。通过RAST抑制试验获得了它们之间过敏原交叉反应性的初步证据。