Nissani Moti
Department of Genetics, University of Wisconsin, 53706, Madison, Wisconsin, USA.
Wilehm Roux Arch Dev Biol. 1977 Sep;182(3):203-211. doi: 10.1007/BF00848341.
Three-hundred and twenty fertile,pal-induced Y-chromosome mosaic males and females were obtained. Fractional analysis of the sons of 55 somatically mosaic flies that were also germinally mosaic tentatively suggests that the number of functional primordial germ cells inDrosophila melanogaster is variable and that it is seldom greater than 24. From the observed 0.17 frequency of germinal mosaicism it was estimated that the average number of pole cells at the end of blastoderm formation is 45. At present, the germ cells afford the only opportunity to compare genetic estimates of the number of blastoderm or primordial cells with available histological counts. The good agreement between them suggests that both the fractional and the mosaic frequency methods for estimating primordial or blastoderm cell numbers of various larval and imaginal anatomical structures provide reasonably close approximations of the actual values.
获得了320只可育的、经PAL诱导的Y染色体嵌合雄性和雌性个体。对55只体细胞嵌合且生殖细胞也嵌合的果蝇的子代进行的分数分析初步表明，黑腹果蝇中功能性原始生殖细胞的数量是可变的，且很少超过24个。根据观察到的0.17的生殖细胞嵌合频率，估计囊胚形成末期极细胞的平均数量为45个。目前，生殖细胞是将囊胚或原始细胞数量的遗传估计值与现有的组织学计数进行比较的唯一机会。它们之间的良好一致性表明，用于估计各种幼虫和成虫解剖结构的原始或囊胚细胞数量的分数法和嵌合频率法都能合理地接近实际值。
