Meyerhof Wolfgang, John Maliyakal E, Thomaschek Gudrun, Knöchel Walter
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimalle 22, D-1000, Berlin.
Wilehm Roux Arch Dev Biol. 1982 Nov;191(6):366-370. doi: 10.1007/BF00879625.
Electron microscopy revealed that transcripts of complementary repetitive sequence elements are widely distributed among long poly (A) containing RNA molecules of Xenopus laevis. cDNA/RNA hybridization experiments suggest that many of these polyadenylated transcripts carry protein coding sequences which are interrupted by repetitive elements. Our in-vitro translation experiments indicate that removal of repetitive elements must precede the utilization of protein coding sequences associated with them. Thus processing of maternal transcripts may be of significance in the regulation of gene expression during early development.
电子显微镜显示，互补重复序列元件的转录本广泛分布于非洲爪蟾含长聚腺苷酸的RNA分子中。cDNA/RNA杂交实验表明，许多这些多聚腺苷酸化的转录本携带被重复元件中断的蛋白质编码序列。我们的体外翻译实验表明，在利用与其相关的蛋白质编码序列之前，必须先去除重复元件。因此，母体转录本的加工在早期发育过程中的基因表达调控中可能具有重要意义。
