Holoubek Viktor, Tiedemann Heinz
Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, Texas, U.S.A.
Institut für Molekularbiologie und Biochemie, Freie Universität, Berlin.
Wilehm Roux Arch Dev Biol. 1978 Jun;184(2):171-180. doi: 10.1007/BF00848225.
The changes in saline-soluble, 0.35 M NaCl-soluble and the residual fraction of nuclear proteins during early development ofXenopus were studied by analytical electrophoresis on sodium dodecyl sulfate polyacrylamide gel. The fractions were obtained by consecutive extraction of nuclei from the blastula, neurula and tail-bud stage of development. No qualitative and only limited quantitative differences were found when the proteins of any of the three fractions isolated from the neurula stage were compared with the proteins of the corresponding fraction isolated from the tail-bud stage. But the electrophoretic pattern of each of the three fractions of the nuclear proteins from the blastula stage differs significantly from the electrophoretic pattern of the same fraction isolated from the neurula or tail-bud stage. Compared with the blastula stage, in the two later stages the relative amounts of chromosomal proteins with apparent molecular weights below 30,000 are decreased. Proteins which migrate in electrophoresis in the positions of the very lysine-rich histones and of the proteins of the nuclear ribonucleo-protein particles are indicated among the chromosomal proteins of the blastula stage, and are visible as strong bands in the electrophorogram of 0.35 M NaCl-soluble proteins extracted from neurula or tail-bud stage nuclei.
通过十二烷基硫酸钠聚丙烯酰胺凝胶分析电泳，研究了非洲爪蟾早期发育过程中盐溶性、0.35M NaCl可溶性及核蛋白残留组分的变化。这些组分是通过从囊胚、神经胚和尾芽期发育阶段的细胞核中连续提取得到的。当比较从神经胚期分离的三个组分中任何一个的蛋白质与从尾芽期分离的相应组分的蛋白质时，未发现定性差异，仅发现有限的定量差异。但是，囊胚期核蛋白的三个组分中每一个的电泳图谱与从神经胚或尾芽期分离的相同组分的电泳图谱有显著差异。与囊胚期相比，在随后的两个阶段中，表观分子量低于30,000的染色体蛋白的相对含量减少。在囊胚期的染色体蛋白中，有在电泳中迁移至富含赖氨酸的组蛋白位置和核糖核蛋白颗粒蛋白位置的蛋白质，并且在从神经胚或尾芽期细胞核中提取的0.35M NaCl可溶性蛋白的电泳图谱中可见为强条带。
