Milos Nadine, Zalik Sara E
Department of Zoology, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada.
Wilehm Roux Arch Dev Biol. 1981 Sep;190(5):259-266. doi: 10.1007/BF00848753.
Cells from the extraembryonic endoderm of the gastrulating chick embryo contain a β-D-galactoside-binding lectin inhibited by thiodigalactoside (TDG). When cell suspensions are cultured in stationary culture in the presence of exogenously added purified blastoderm lectin or TDG, their attachment to the substratum is delayed and decreased compared to controls. The cells take on a fibroblastic-like morphology and cell to cell contact becomes limited to localized areas of the cell surface. Many lectin or TDG-treated cells appear to be migrating over the substratum. This is in contrast to control cultures where the cells appear epithelial in morphology and tend to maximize their areas of apposition. These data suggest that the endogenous lectin may have a role to play in cell to substratum and cell to cell adhesion.
处于原肠胚形成期的鸡胚胚外内胚层细胞含有一种受硫代二半乳糖苷（TDG）抑制的β-D-半乳糖苷结合凝集素。当细胞悬液在添加了外源性纯化胚盘凝集素或TDG的静止培养条件下培养时，与对照组相比，它们附着于基质的过程延迟且附着量减少。细胞呈现出成纤维细胞样形态，细胞间接触局限于细胞表面的局部区域。许多经凝集素或TDG处理的细胞似乎在基质上迁移。这与对照培养不同，对照培养中的细胞形态呈上皮样，且倾向于使它们的附着面积最大化。这些数据表明内源性凝集素可能在细胞与基质以及细胞与细胞的黏附中发挥作用。
