Zalik S E, Milos N, Ledsham I
Cell Tissue Res. 1982;225(1):223-8. doi: 10.1007/BF00216231.
Cells from the endoderm of the area opaca of gastrulating chick embryos were maintained in stationary cultures, stained with antibodies against the endogenous beta-D-galactoside-binding lectin and examined by immunofluorescence. In the majority of cells fluorescence was present as an irregular circular web in the central cytoplasm. In cells that appeared to be migrating increased fluorescence was observed in the peripheral cytoplasm and retraction fibers. In regions where a portion of the cell was detaching from the substratum high fluorescence was observed in the extracellular "footprints" deposited by the cell.
将原肠胚期鸡胚暗区内胚层的细胞进行静止培养，用抗内源性β-D-半乳糖苷结合凝集素的抗体染色，然后通过免疫荧光法进行检测。在大多数细胞中，荧光呈现为中央细胞质中不规则的圆形网络。在似乎正在迁移的细胞中，外周细胞质和收缩纤维中观察到荧光增强。在细胞的一部分与基质分离的区域，在细胞沉积的细胞外“足迹”中观察到高荧光。
