来自凹顶伪球海胆卵巢的纤连蛋白。

Fibronectin from the ovary of the sea urchin,Pseudocentrotus depressus.

作者信息

Iwata Mineo, Nakano Eizo

机构信息

Department of Biology, Nagoya University, Chikusa, 464, Nagoya, Japan.

出版信息

Wilehm Roux Arch Dev Biol. 1981 Mar;190(2):83-86. doi: 10.1007/BF00848399.

DOI:10.1007/BF00848399

PMID:28305355

Abstract

Fibronectin, with a subunit molecular weight of 220,000 daltons, was isolated from the ovary of the sea urchin,Pseudocentrotus depressus, using affinity chromatography on heat-denatured mammalian collagen coupled to Sepharose 4B. The distribution of fibronectin in the sea urchin ovary was examined by indirect immunofluorescence using antifibronectin serum. The basement membrane and the connective tissues exhibited strong fluorescence. The fibronectin was localized closely together with collagen bundles in the sea urchin ovary. Biochemical and immunological examinations indicate that sea urchin fibronectin has similar properties as those of mammalian fibronectin.

摘要

纤连蛋白的亚基分子量为220,000道尔顿，它是从海胆（Pseudocentrotus depressus）的卵巢中分离出来的，采用了在与琼脂糖凝胶4B偶联的热变性哺乳动物胶原蛋白上进行亲和层析的方法。利用抗纤连蛋白血清，通过间接免疫荧光法检测了纤连蛋白在海胆卵巢中的分布。基底膜和结缔组织呈现出强烈的荧光。在海胆卵巢中，纤连蛋白与胶原束紧密定位在一起。生化和免疫学检测表明，海胆纤连蛋白具有与哺乳动物纤连蛋白相似的特性。