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斯氏摇蚊(双翅目:摇蚊科)低紫外线剂量双腹诱导:敏感期与完全光可逆性

Double abdomen induction with low UV-doses inSmittia spec. (Chironomidae, diptera): Sensitive period and complete photoreversibility.

作者信息

Ripley Susan, Kalthoff Klaus

机构信息

Department of Zoology, University of Texas, 78712, Austin, Texas, USA.

出版信息

Wilehm Roux Arch Dev Biol. 1981 Jan;190(1):49-54. doi: 10.1007/BF00868703.

DOI:10.1007/BF00868703
PMID:28305370
Abstract

In embryos of the chironomid midgeSmittia spec., UV-irradiation of anterior pole regions results in double abdomen formation. In this abnormal body pattern, head and thorax are replaced by a mirror image of the abdomen. Irradiation at a particular stage between nuclear migration and blastoderm formation, and with the anterior pole facing the UV-beam, yields 100% double abdomens after a minimum UV-dose (140 J·m at 285 nm wavelength). By subsequent exposure to light of longer wavelength, embryos can be reprogrammed so that they develop normally again. The irradiation procedure described is suitable for programming large numbers of embryos for double abdomen formation, with a minimum of side effects and virtually complete photoreversibility.

摘要

在摇蚊属的斯氏摇蚊胚胎中,前极区域的紫外线照射会导致双腹形成。在这种异常的身体模式中,头部和胸部被腹部的镜像所取代。在核迁移和胚盘形成之间的特定阶段进行照射,且前极朝向紫外线束,在最小紫外线剂量(波长285纳米时为140焦耳·平方米)后可产生100%的双腹。通过随后暴露于波长更长的光下,胚胎可以重新编程,从而再次正常发育。所描述的照射程序适用于对大量胚胎进行双腹形成的编程,副作用最小且几乎完全具有光可逆性。

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1
Double abdomen induction with low UV-doses inSmittia spec. (Chironomidae, diptera): Sensitive period and complete photoreversibility.斯氏摇蚊(双翅目:摇蚊科)低紫外线剂量双腹诱导:敏感期与完全光可逆性
Wilehm Roux Arch Dev Biol. 1981 Jan;190(1):49-54. doi: 10.1007/BF00868703.
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Changes in the apparent localization of anterior determinants during early embryogenesis (Smittia spec., Chironomidae, Diptera).早期胚胎发育过程中前决定因子表观定位的变化(斯氏摇蚊,摇蚊科,双翅目)
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本文引用的文献

1
Temperature effects on embryogenesis in Smittia spec. (Diptera, chironomidae): Q-values of normal development and frequency of "double abdomens" after UV irradiation.温度对斯氏摇蚊(双翅目,摇蚊科)胚胎发生的影响:正常发育的Q值及紫外线照射后“双腹”的频率
Wilhelm Roux Arch Entwickl Mech Org. 1971 Jun;168(2):85-96. doi: 10.1007/BF00581802.
2
Position of targets and period of competence for UV-induction of the malformation "Double Abdomen" in the egg ofSmittia spec. (Diptera, Chironomidae).斯氏摇蚊(双翅目,摇蚊科)卵中“双腹”畸形紫外线诱导的靶标位置及感受期
Wilhelm Roux Arch Entwickl Mech Org. 1971 Mar;168(1):63-84. doi: 10.1007/BF00582004.
3
昆虫(中间红蝽,半翅目,红蝽科)卵子发生和早期发育过程中核糖体后颗粒的免疫荧光分布
Wilehm Roux Arch Dev Biol. 1983 Mar;192(2):53-61. doi: 10.1007/BF00848481.
4
Scanning electron microscopic analysis of spontaneous and UV-induced abnormal segment patterns in Chironomus samoensis (Diptera, Chironomidae).萨摩按蚊(双翅目,摇蚊科)自发及紫外线诱导的异常节段模式的扫描电子显微镜分析
Rouxs Arch Dev Biol. 1986 Feb;195(2):92-102. doi: 10.1007/BF00456105.
5
Changes in the apparent localization of anterior determinants during early embryogenesis (Smittia spec., Chironomidae, Diptera).早期胚胎发育过程中前决定因子表观定位的变化(斯氏摇蚊,摇蚊科,双翅目)
Wilehm Roux Arch Dev Biol. 1983 Nov;192(6):353-361. doi: 10.1007/BF00848816.
[Development of the malformation "double abdomen" in eggs of smittia parthenogenetica (dipt., chironomidae) partially irradiated by UV].
[紫外线部分照射致孤雌生殖直突摇蚊(双翅目,摇蚊科)卵中“双腹”畸形的发育]
Wilhelm Roux Arch Entwickl Mech Org. 1968 Jun;161(2):129-146. doi: 10.1007/BF00585968.
4
STUDIES ON EMBRYONIC DETERMINATION OF THE HARLEQUIN-FLY, CHIRONOMUS DORSALIS. II. EFFECTS OF PARTIAL IRRADIATION OF THE EGG BY ULTRA-VIOLET LIGHT.背摇蚊胚胎决定的研究。II. 紫外线对卵的局部照射的影响。
J Embryol Exp Morphol. 1964 Mar;12:89-100.
5
Studies on embryonic determination of the harlequin-fly, Chironomus dorsalis. I. Effects of centrifugation and of its combination with constriction and puncturing.背摇蚊胚胎决定作用的研究。I. 离心及其与缢缩和穿刺相结合的影响。
J Embryol Exp Morphol. 1960 Jun;8:198-215.
6
Photoreversible UV-inactivation of messenger RNA in an insect embryo(Smittia spec., Chironomidae, Diptera).昆虫胚胎(摇蚊科摇蚊属,双翅目)中信使核糖核酸的光可逆紫外线失活
Photochem Photobiol. 1980 Dec;32(6):749-61. doi: 10.1111/j.1751-1097.1980.tb04052.x.
7
Proteins foretelling head or abdomen development in the embryo of Smittia spec. (Chironomidae, Diptera).斯氏摇蚊(双翅目:摇蚊科)胚胎中预示头部或腹部发育的蛋白质。
Dev Biol. 1981 Jul 30;85(2):287-98. doi: 10.1016/0012-1606(81)90260-8.
8
Synthesis of a posterior indicator protein in normal embryos and double abdomens of Smittia sp. (Chironomidae, Diptera).正常胚胎及斯氏摇蚊(双翅目:摇蚊科)双腹个体中一种后部指示蛋白的合成
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6700-4. doi: 10.1073/pnas.77.11.6700.
9
Photoreversion of UV induction of the malformation "double abdomen" in the egg of Smittia spec. (Diptera, Chironomidae).斯氏摇蚊(双翅目,摇蚊科)卵中紫外线诱导的“双腹”畸形的光逆转
Dev Biol. 1971 May;25(1):119-32. doi: 10.1016/0012-1606(71)90022-4.
10
Photosensitized formation of RNA-protein crosslinks in an insect egg (Smittia spec., Chironomidae, Diptera).
Photochem Photobiol. 1979 May;29(5):1039-40. doi: 10.1111/j.1751-1097.1979.tb07811.x.