Perondini A L P, Gutzeit H O, Sander K
Departamento de Biologia, Institute de Biociencias, Universidade de São Paulo, CP 11461, São Paulo, Brazil.
Institut für Biologie I (Zoologie), Universität Freiburg, Albertstraße 21 A, D-7800, Freiburg, Germany.
Rouxs Arch Dev Biol. 1987 Apr;196(4):268-272. doi: 10.1007/BF00376352.
The pattern anomaly double abdomen was induced in embryos of Bradysia tritici (syn. Sciara ocellaris) by irradiation of the anterior egg pole with far UV (254 or 285 nm) using low UV fluences. The maximum yield of 18% of double abdomens was obtained when 2.5 h embryos were irradiated (late intravitelline cleavage stage); earlier irradiation failed to yield double abdomens, as did irradiations after the early syncytial blastoderm stage. Exposing irradiated embryos to photoreverting light (366 nm) reduced the yield of malformations. Most double abdomens were symmetrical and the number of segments ranged from 3 to 8 in each set, with the mean value at 6.4 segments.
利用低紫外线通量，用远紫外线（254或285纳米）照射小麦尖眼蕈蚊（同物异名：眼菌蚊）胚胎的卵前端，诱导出模式异常的双腹现象。当照射2.5小时龄的胚胎（卵内晚期卵裂阶段）时，双腹的最大发生率为18%；早期照射未能产生双腹现象，早期合胞体胚盘阶段之后的照射也是如此。将受照射的胚胎暴露于光逆转光（366纳米）下，可降低畸形发生率。大多数双腹是对称的，每组的节段数在3至8个之间，平均值为6.4节段。
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