Brown P M, Kalthoff K
Dev Biol. 1983 May;97(1):113-22. doi: 10.1016/0012-1606(83)90069-6.
The formation of pole cells (primordial germ cells) in Smittia sp can be inhibited by ultraviolet (uv) irradiation without causing significant mortality. Until 70 min after egg deposition, pole cells are suppressed by low uv doses applied to the posterior pole region. Microbeam irradiation of a target area including the oosome inhibits pole cell formation; this is not observed after irradiation of other target areas. The action spectrum for uv inhibition of pole cells shows a distinct peak at 260 nm; its shape suggests that a nucleic acid-protein complex acts as an effective target. Independent evidence for the involvement of a nucleic acid moiety is derived from the fact that uv inhibition of pole cell formation is photoreversible. The results are discussed in the context of pole cell determination by localized cytoplasmic components.
在斯氏摇蚊中，极细胞（原始生殖细胞）的形成可被紫外线（uv）照射抑制，且不会导致显著的死亡率。在卵产下后70分钟内，施加于后极区域的低剂量紫外线会抑制极细胞的形成。对包括卵核在内的目标区域进行微束照射会抑制极细胞的形成；而对其他目标区域照射后则未观察到这种现象。紫外线抑制极细胞形成的作用光谱在260纳米处有一个明显的峰值；其形状表明核酸 - 蛋白质复合物是有效的靶标。核酸部分参与其中的独立证据来自于紫外线对极细胞形成的抑制是可光逆转的这一事实。我们将在由局部细胞质成分决定极细胞的背景下讨论这些结果。
