Matsuda Motoko, Onitake Kazuo
Biological Institute, Faculty of Science, Nagoya University, 464, Nagoya, Japan.
Biological Laboratory, Nagoya University College of Medical Technology, Daiko-cho, 461, Nagoya.
Wilehm Roux Arch Dev Biol. 1984 Mar;193(2):61-63. doi: 10.1007/BF00848631.
Cynops pyrrhogaster oviducal eggs with and without jelly envelopes (jelly egg and dejellied egg respectively) were immersed in water, and then inseminated artificially. After 1 h of immersion in water, more than half the dejellied eggs were fertilized and developed, but no jelly eggs developed. The rapid decrease in the ability of jelly eggs to be fertilized after immersion in water is not due to a deficiency in the egg. Our results make it clear that hydrated jelly envelopes prevent the eggs from fertilizing. The ability of the egg to be fertilized decreases for a long time in water and this decrease proceeds more slowly in De Boer's solution or Holtfreter's balanced salt solution than in water.
将有或没有卵胶膜（分别为带胶膜卵和去胶膜卵）的日本林蛙输卵管卵浸入水中，然后进行人工授精。在水中浸泡1小时后，超过一半的去胶膜卵受精并发育，但带胶膜卵未发育。带胶膜卵在水中浸泡后受精能力迅速下降并非由于卵本身存在缺陷。我们的结果表明，水合的卵胶膜会阻止卵受精。卵在水中的受精能力会在很长一段时间内下降，并且这种下降在德布尔溶液或霍尔特弗雷ter平衡盐溶液中比在水中进行得更慢。
