Mclaughlin E W, Humphries A A
J Morphol. 1978 Oct;158(1):73-90. doi: 10.1002/jmor.1051580107.
Fertilization in Notophthalmus viridescens is internal and involves passage of the sperm through five layers of egg jelly (J5-J1, from outermost to innermost), each of which is secreted by a discrete region of the oviduct. Polyspermy is normal. Passage of the sperm through the jelly and into the egg was studied by a technique of artificial insemination similar to natural insemination, in that undiluted fluid from the vas deferens was applied directly to eggs with various layers of jelly present, followed by flooding with water three to five minutes later. In general, successful fertilization increased as the number of jelly layers increased; jellyless coelomic eggs were not fertilizable. Sperm passage through the jelly and into the egg usually occurs within one to three minutes. Upon hydration of the jelly, barriers to sperm penetration develop in layers J5 and J3. Changes in the egg jelly thus seem to be involved in the restriction of polyspermy to a low level.
绿红东美螈的受精过程是体内受精，精子需穿过五层卵胶膜（从最外层到最内层依次为J5 - J1），每层卵胶膜由输卵管的不同区域分泌。多精入卵现象很常见。通过一种类似于自然授精的人工授精技术研究了精子穿过卵胶膜进入卵子的过程，即将来自输精管的未稀释液体直接施加到带有不同层数卵胶膜的卵子上，三到五分钟后用水冲洗。一般来说，随着卵胶膜层数的增加，成功受精的几率也会增加；没有卵胶膜的体腔卵无法受精。精子穿过卵胶膜进入卵子通常在一到三分钟内发生。卵胶膜水化后，J5层和J3层会形成精子穿透的屏障。因此，卵胶膜的变化似乎与将多精入卵限制在低水平有关。
