Engström Ylva, Schneuwly Stephan, Gehring Walter Jakob
Department of Molecular Biology, University of Stockholm, S-106 91, Stockholm, Sweden.
Institut für Genetik und Mikrobiologie der Universität Würzburg, Röntgenring 11, D-8700, Würzburg, Germany.
Rouxs Arch Dev Biol. 1992 Apr;201(2):65-80. doi: 10.1007/BF00420417.
In order to study the regulation of spatial and temporal expression of the homeotic gene Antennapedia (Antp) in Drosophila melanogaster, we have constructed fusion genes which contain Antp sequences linked to the reporter gene lac Z of Escherichia coli. In one case of P-element transformation, a fusion gene construct integrated into the endogenous Antp gene close to one of the two promoters (P1). The spatial expression from the reporter gene in this transformant line, as analysed by the detection of β-galactosidase activity, was found to exactly mimic the normal expression from the P1 promoter of the Antp gene. We have used this unique transformant as a tool for studying the expression of the P1 promoter in embryonic, larval and adult development. Parallel lines transformed with the same fusion gene construct did not confer a correct P1 pattern of expression. The position in the genome was, therefore, crucial for the expression pattern of the reporter gene. Experiments aiming at the detection of autoregulatory control of Antp gene expression were designed. The results did not, however, support models of positive or negative autoregulation of P1 expression by Amp protein.
为了研究黑腹果蝇中同源异型基因触角足基因（Antp）的时空表达调控，我们构建了融合基因，其包含与大肠杆菌报告基因lac Z相连的Antp序列。在一种P元件转化的情况下，一个融合基因构建体整合到内源性Antp基因中，靠近两个启动子（P1）之一。通过检测β-半乳糖苷酶活性分析，该转化株系中报告基因的空间表达被发现精确模拟了Antp基因P1启动子的正常表达。我们已将这个独特的转化株用作研究P1启动子在胚胎、幼虫和成虫发育中表达的工具。用相同融合基因构建体转化的平行株系并未赋予正确的P1表达模式。因此，基因组中的位置对于报告基因的表达模式至关重要。设计了旨在检测Antp基因表达的自调控控制的实验。然而，结果并不支持Amp蛋白对P1表达进行正调控或负调控的模型。
