Boulet A M, Scott M P
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80302.
Genes Dev. 1988 Dec;2(12A):1600-14. doi: 10.1101/gad.2.12a.1600.
Antennapedia (Antp), a homeotic gene of Drosophila required for proper differentiation of the thorax of the fly, is expressed in complex spatial patterns during development. The gene is greater than 100 kb long and has two independently regulated promoters. To characterize cis-acting regulatory elements responsible for the expression pattern, fusions of the Antp promoter 2 cap site and upstream sequences to an Adh-lacZ gene were introduced into flies. A 10-kb sequence directs beta-galactosidase production in a pattern that closely resembles the endogenous P2 pattern. Transcription from the 10-kb fusions is regulated by three genes that regulate Antp transcription. Control elements, including a target of action of homeo-domain-containing proteins, were mapped by deleting parts of the 10-kb sequence.
触角足基因（Antp）是果蝇的一个同源异型基因，对果蝇胸部的正常分化是必需的，在发育过程中以复杂的空间模式表达。该基因长度超过100 kb，有两个独立调控的启动子。为了鉴定负责这种表达模式的顺式作用调控元件，将Antp启动子2帽位点及上游序列与Adh-lacZ基因的融合体导入果蝇。一个10 kb的序列以与内源性P2模式非常相似的模式指导β-半乳糖苷酶的产生。来自10 kb融合体的转录受三个调控Antp转录的基因调控。通过缺失10 kb序列的部分片段，绘制了包括含同源结构域蛋白作用靶点在内的调控元件图谱。
