曲古抑菌素A诱导的组蛋白多乙酰化形式的积累及其对海星早期胚胎发育的影响

Accumulation of multiacetylated forms of histones by trichostatin A and its developmental consequences in early starfish embryos.

作者信息

Ikegami Susumu, Ooe Yasunori, Shimizu Takahiko, Kasahara Toshihiko, Tsuruta Tatsuhiko, Kijima Masako, Yoshida Minoru, Beppu Teruhiko

机构信息

Department of Applied Biochemistry, Hiroshima University, 4-4, Kagamiyama 1 Chome, 724, Higashi-Hiroshima, Hiroshima, Japan.

Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Yayoi 1-1-1, 113, Bunkyo-ku, Tokyo, Japan.

出版信息

Rouxs Arch Dev Biol. 1993 Feb;202(3):144-151. doi: 10.1007/BF00365304.

DOI:10.1007/BF00365304

PMID:28305991

Abstract

External application of 10 rig/ml (R)-trichostatin A (TSA), a potent and specific inhibitor of mammalian histone deacetylase, to the embryo of the starfish Asterina pectinifera inhibited development during the early gastrula stage before formation of mesenchyme cells. The TSA-sensitive period was limited to the mid-blastula stage before hatching. The pulse-chase experiment clearly demonstrated that TSA induced an accumulation of acetylated histone species in blastulae through inhibition of historic deacetylation. Similar blockage of development at the early gastrula stage was observed with n-butyrate, which has been known as a weak inhibitor of historic deacetylase. These results suggest an intimate role for historic acetylation-deacetylation equilibria in starfish development.

摘要

将10 rig/ml的（R）-曲古抑菌素A（TSA）（一种强效且特异性的哺乳动物组蛋白脱乙酰酶抑制剂）外用至栉孔扇贝海星胚胎上，会在间充质细胞形成之前的原肠胚早期阶段抑制发育。TSA敏感时期局限于孵化前的囊胚中期阶段。脉冲追踪实验清楚地表明，TSA通过抑制组蛋白去乙酰化诱导囊胚中乙酰化组蛋白种类的积累。用已知为组蛋白脱乙酰酶弱抑制剂的丁酸盐在原肠胚早期阶段观察到了类似的发育阻滞。这些结果表明组蛋白乙酰化-去乙酰化平衡在海星发育中起着密切作用。