Yamada H, Hirai S, Ikegami S, Kawarada Y, Okuhara E, Nagano H
J Cell Physiol. 1985 Jul;124(1):9-12. doi: 10.1002/jcp.1041240103.
In fertilized echinoderm eggs, the male and female pronuclei fuse to form the diploid nucleus even in the presence of aphidicolin, a specific inhibitor of eukaryotic DNA polymerase-alpha. The subsequent first cleavage is independent of chromosomes but dependent on spindle and amphiaster. The fate of DNA originally existing in the fused nucleus during achromosomal cleavage of fertilized sea urchin and starfish eggs induced by aphidicolin was determined using antidenatured DNA antibody. The nucleus is not formed in the divided daughter cells at the two-cell stage but the nuclear-envelope-free chromatin mass which is unassociated with mitotic apparatus remains in the center region of embryos, especially near the first cleavage furrow. These results indicate that the condensed and nonreplicated chromatin can not be associated with spindle and asters in the presence of aphidicolin.
在受精的棘皮动物卵中，即使存在真核生物DNA聚合酶α的特异性抑制剂阿非科林，雄原核和雌原核也会融合形成二倍体细胞核。随后的第一次卵裂独立于染色体，但依赖于纺锤体和星体。使用抗变性DNA抗体确定了在阿非科林诱导的受精海胆和海星卵的无染色体卵裂过程中，融合细胞核中原本存在的DNA的命运。在二细胞期的分裂子细胞中不形成细胞核，但与有丝分裂装置无关的无核膜染色质团块留在胚胎的中心区域，特别是靠近第一次卵裂沟的地方。这些结果表明，在存在阿非科林的情况下，浓缩且未复制的染色质不能与纺锤体和星体相关联。
