Gorman C M, Howard B H, Reeves R
Nucleic Acids Res. 1983 Nov 11;11(21):7631-48. doi: 10.1093/nar/11.21.7631.
We have studied the effects of sodium butyrate on DNA-mediated gene transfer in an effort to investigate interrelationships between chromatin structure and expression of recombinant plasmids. Our results demonstrate that butyrate affects the early stages of gene activity following DNA uptake at least two levels. First, the number of cells able to express foreign DNA increases from 10% to up to 40%. Second, there is an increase in enhancer-dependent transcription, approximately 30 fold in HeLa cells, involving the SV40 early promoter. Stable transformation efficiencies increase to 4% and 10% in HeLa S3 and monkey kidney CV-1 cells, respectively. Finally, expression of integrated recombinant plasmid genes is reinducible by a second treatment five weeks after initial exposure to this agent.
我们研究了丁酸钠对DNA介导的基因转移的影响，旨在探究染色质结构与重组质粒表达之间的相互关系。我们的结果表明，丁酸钠至少在两个水平上影响DNA摄取后基因活性的早期阶段。首先，能够表达外源DNA的细胞数量从10%增加到高达40%。其次，增强子依赖性转录增加，在HeLa细胞中约为30倍，涉及SV40早期启动子。在HeLa S3细胞和猴肾CV-1细胞中，稳定转化效率分别提高到4%和10%。最后，在初次接触该试剂五周后，通过第二次处理可再次诱导整合的重组质粒基因的表达。
