Nelson Heidi B, Laughon Allen
Program of Cellular and Molecular Biology, University of Wisconsin-Madison, 53706, Madison, WI, USA.
Laboratory of Genetics, University of Wisconsin-Madison, 53706, Madison, WI, USA.
Rouxs Arch Dev Biol. 1993 Aug;202(6):341-354. doi: 10.1007/BF00188733.
Monoclonal antibodies and enhancer trap insertions that mark subsets of neurons have been valuable tools in the study of Drosophila neuronal development. Similarly, glial specific cell markers could prove to be valuable in investigating the development and function of glia. Here we characterize the architecture and development of distinct sets of Drosophila embryonic glia, using a reporter gene driven by fushi tarazu homeodomain binding sites. Reporter expresssion in glia is dependent on the orientation and spacing of the homeodomain binding sites, revealing potential differences in glial determination. These studies suggest that the use of transcription factor binding sites to drive reporter gene expression may prove to be a generally useful means of generating additional cell type-specific markers.
标记神经元亚群的单克隆抗体和增强子陷阱插入在果蝇神经元发育研究中一直是有价值的工具。同样，胶质细胞特异性细胞标记物可能在研究胶质细胞的发育和功能方面被证明是有价值的。在这里，我们利用由fushi tarazu同源结构域结合位点驱动的报告基因，对果蝇胚胎胶质细胞不同组的结构和发育进行了表征。报告基因在胶质细胞中的表达取决于同源结构域结合位点的方向和间距，揭示了胶质细胞决定中的潜在差异。这些研究表明，利用转录因子结合位点来驱动报告基因表达可能被证明是产生额外细胞类型特异性标记物的一种普遍有用的方法。
