Skuce P J, Anderson H R, Fairweather I
Department of Zoology, Queen's University, Belfast, Northern Ireland.
Parasitol Res. 1987;74(2):161-7. doi: 10.1007/BF00536028.
The relative effects on tegumental Na+/K+-ATPase activity in Fasciola hepatica of the deacetylated (amine) metabolite of diamphenethide (DAMD) (10 micrograms/ml, 18 h) and the Na+/K+-ATPase inhibitor ouabain (0.1 mM, 0.5 h) have been assessed cytochemically. In the normal tegument, Na+/K+-ATPase activity is particularly concentrated along the invaginations of the apical plasma membrane and the infoldings of the basal plasma membrane. Ouabain pretreatment significantly reduces the overall level of Na+/K+-ATPase activity, but does not induce swelling of the basal infolds. In contrast, DAMD does not significantly reduce either the level or distribution of Na+/K+-ATPase activity, but does cause a pronounced swelling of the basal infolds. The results are discussed in relation to the postulated action of diamphenethide as an inhibitor of Na+/K+-ATPase activity.
已通过细胞化学方法评估了双胺苯硫醚（DAMD）的脱乙酰化（胺）代谢产物（10微克/毫升，18小时）和Na+/K+-ATP酶抑制剂哇巴因（0.1毫摩尔，0.5小时）对肝片吸虫体表Na+/K+-ATP酶活性的相对影响。在正常体表中，Na+/K+-ATP酶活性特别集中在顶端质膜的内陷处和基底质膜的褶皱处。哇巴因预处理显著降低了Na+/K+-ATP酶活性的总体水平，但不会引起基底褶皱肿胀。相比之下，DAMD既不会显著降低Na+/K+-ATP酶活性的水平或分布，但确实会导致基底褶皱明显肿胀。结合双胺苯硫醚作为Na+/K+-ATP酶活性抑制剂的假定作用对结果进行了讨论。
