CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
Shanghai University of Medicine & Health Sciences , Shanghai 200120, China.
ACS Appl Mater Interfaces. 2017 Apr 12;9(14):12217-12226. doi: 10.1021/acsami.6b16705. Epub 2017 Mar 28.
This is high demand to enhance the accumulation of near-infrared theranostic agents in the tumor region, which is favorable to the effective phototherapy. Compared with indocyanine green (a clinically applied dye), IR-780 iodide possesses higher and more stable fluorescence intensity and can be utilized as an imaging-guided PTT agent with laser irradiation. However, lipophilicity and short circulation time limit its applications in cancer imaging and therapy. Moreover, solid lipid nanoparticles (SLNs) conjugated with c(RGDyK) was designed as efficient carriers to improve the targeted delivery of IR-780 to the tumors. The multifunctional cRGD-IR-780 SLNs exhibited a desirable monodispersity, preferable stability and significant targeting to cell lines overexpressing αβ integrin. Additionally, the in vitro assays such as cell viability and in vivo PTT treatment denoted that U87MG cells or U87MG transplantation tumors could be eradicated by applying cRGD-IR-780 SLNs under laser irradiation. Therefore, the resultant cRGD-IR-780 SLNs may serve as a promising NIR imaging-guided targeting PTT agent for cancer therapy.
这就需要提高近红外治疗剂在肿瘤部位的积累，这有利于有效的光疗。与吲哚菁绿（一种临床应用的染料）相比，IR-780 碘化物具有更高、更稳定的荧光强度，可用作激光照射的成像引导 PTT 剂。然而，亲脂性和短循环时间限制了其在癌症成像和治疗中的应用。此外，设计了与 c(RGDyK) 缀合的固体脂质纳米粒 (SLNs) 作为有效的载体，以提高 IR-780 对肿瘤的靶向递送。多功能 cRGD-IR-780 SLNs 表现出良好的单分散性、更好的稳定性和对过度表达 αβ 整合素的细胞系的显著靶向性。此外，体外细胞活力测定和体内 PTT 治疗表明，在激光照射下，U87MG 细胞或 U87MG 移植瘤可以被 cRGD-IR-780 SLNs 消除。因此，所得的 cRGD-IR-780 SLNs 可用作癌症治疗中具有前景的近红外成像引导靶向 PTT 剂。
