Kenney S, Kamine J, Markovitz D, Fenrick R, Pagano J
Lineberger Cancer Research Center, Department of Medicine, University of North Carolina, Chapel Hill 27514.
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1652-6. doi: 10.1073/pnas.85.5.1652.
Acquired immunodeficiency syndrome patients are frequently coinfected with Epstein-Barr virus (EBV). In this report, we demonstrate that an EBV immediate-early gene product, BamHI MLF1, stimulates expression of the bacterial chloramphenicol acetyltransferase (CAT) gene linked to the human immunodeficiency virus (HIV) promoter. The HIV promoter sequences necessary for trans-activation by EBV do not include the tat-responsive sequences. In addition, in contrast to the other herpesvirus trans-activators previously studied, the EBV BamHI MLF1 gene product appears to function in part by a posttranscriptional mechanism, since it increases pHIV-CAT protein activity more than it increases HIV-CAT mRNA. This ability of an EBV gene product to activate HIV gene expression may have biologic consequences in persons coinfected with both viruses.
获得性免疫缺陷综合征患者常合并感染爱泼斯坦-巴尔病毒（EBV）。在本报告中，我们证明EBV的一种立即早期基因产物BamHI MLF1可刺激与人类免疫缺陷病毒（HIV）启动子相连的细菌氯霉素乙酰转移酶（CAT）基因的表达。EBV反式激活HIV所需的启动子序列不包括tat反应序列。此外，与先前研究的其他疱疹病毒反式激活因子不同，EBV BamHI MLF1基因产物似乎部分通过转录后机制发挥作用，因为它增加pHIV-CAT蛋白活性的程度大于增加HIV-CAT mRNA的程度。EBV基因产物激活HIV基因表达的这种能力可能对同时感染这两种病毒的人产生生物学影响。
