Kenney S, Kamine J, Holley-Guthrie E, Mar E C, Lin J C, Markovitz D, Pagano J
Lineberger Cancer Research Center, University of North Carolina, Chapel Hill 27514.
J Virol. 1989 Sep;63(9):3870-7. doi: 10.1128/JVI.63.9.3870-3877.1989.
In DNA cotransfection experiments, the Epstein-Barr virus immediate-early gene product, BMLF1, stimulated the chloramphenicol acetyltransferase (CAT) activity of both latent and productive EBV promoters linked to CAT. This BMLF1-induced increase in CAT activity was out of proportion to the effect on CAT mRNA, suggesting a posttranscriptional mechanism. Furthermore, when growth hormone was used as a reporter gene instead of CAT, BMLF1 no longer functioned. Thus, the BMLF1 effect was reporter-gene dependent. The effect of the BMLF1 gene product does not then appear to be directed at promoter activation, but instead may function to increase the level of an as yet unidentified protein(s) required for Epstein-Barr virus infection.
在DNA共转染实验中，爱泼斯坦-巴尔病毒即刻早期基因产物BMLF1刺激了与氯霉素乙酰转移酶（CAT）相连的潜伏性和增殖性EBV启动子的CAT活性。BMLF1诱导的CAT活性增加与对CAT mRNA的影响不成比例，提示存在转录后机制。此外，当使用生长激素代替CAT作为报告基因时，BMLF1不再发挥作用。因此，BMLF1的作用依赖于报告基因。BMLF1基因产物的作用似乎并非针对启动子激活，而是可能起到增加爱泼斯坦-巴尔病毒感染所需的一种尚未确定的蛋白质水平的作用。
