Davis M G, Kenney S C, Kamine J, Pagano J S, Huang E S
Lineberger Cancer Research Center, University of North Carolina, Chapel Hill 27514.
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8642-6. doi: 10.1073/pnas.84.23.8642.
Almost all homosexual patients with acquired immunodeficiency syndrome are also actively infected with human cytomegalovirus (HCMV). We have hypothesized that an interaction between HCMV and human immunodeficiency virus (HIV), the agent that causes acquired immunodeficiency syndrome, may exist at a molecular level and contribute to the manifestations of HIV infection. In this report, we demonstrate that the immediate-early gene region of HCMV, in particular immediate-early region 2, trans-activates the expression of the bacterial gene chloramphenicol acetyltransferase that is fused to the HIV long terminal repeat and carried by plasmid pHIV-CAT. The HCMV immediate-early trans-activator increases the level of mRNA from the plasmid pHIV-CAT. The sequences of HIV that are responsive to trans-activation by the HCMV immediate-early region are distinct from HIV sequences that required for response to the HIV tat. The stimulation of HIV gene expression by HCMV gene functions could enhance the consequences of HIV infection in persons with previous or concurrent HCMV infection.
几乎所有患获得性免疫缺陷综合征的同性恋患者也都同时受到人巨细胞病毒（HCMV）的活跃感染。我们推测，HCMV与导致获得性免疫缺陷综合征的病原体——人类免疫缺陷病毒（HIV）之间可能在分子水平上存在相互作用，并促使HIV感染症状的出现。在本报告中，我们证明HCMV的立即早期基因区域，尤其是立即早期区域2，可反式激活与HIV长末端重复序列融合并由质粒pHIV-CAT携带的细菌氯霉素乙酰转移酶基因的表达。HCMV立即早期反式激活因子可提高来自质粒pHIV-CAT的mRNA水平。对HCMV立即早期区域反式激活有反应的HIV序列，与对HIV反式激活应答元件（tat）有反应所需的HIV序列不同。HCMV基因功能对HIV基因表达的刺激，可能会加重既往或同时感染HCMV者的HIV感染后果。
