Piqueras Paloma, Gallardo Miguel, Hebles María, Jiménez Juan Manuel, Migueles Beatriz, Montero Lorena, Sánchez-Martín Fernando, Sánchez-Martín Pascual
a Clínicas Ginemed Sevilla , Seville , Spain.
b Clínicas Ginemed , Lisboa , Lisbon , Portugal.
Syst Biol Reprod Med. 2017 Jun;63(3):209-211. doi: 10.1080/19396368.2017.1285371. Epub 2017 Mar 17.
This case report describes a live birth after the fresh replacement of an embryo obtained from a spontaneously in vitro matured oocyte. The patient was subjected to controlled ovarian stimulation for IVF treatment, obtaining two oocytes. One was found to be immature at the time of denudation, at metaphase-I. This immature oocyte was kept in culture overnight in standard conditions along with the second oocyte - which was mature but failed to fertilize - spontaneously achieving metaphase-II, and was subjected to ICSI. The resulting embryo was replaced on the second day of development, producing a pregnancy that resulted in a healthy live birth. Post-denudation in vitro maturation could be considered as a tool to improve reproductive outcomes in selected patients, such as poor responders.
本病例报告描述了一例使用经自发体外成熟卵母细胞获得的胚胎进行新鲜移植后活产的案例。该患者接受了用于体外受精治疗的控制性卵巢刺激，获得了两个卵母细胞。其中一个在剥除时被发现不成熟，处于减数分裂中期I。这个未成熟卵母细胞与第二个卵母细胞一起在标准条件下培养过夜，第二个卵母细胞是成熟的但未受精，它自发地达到了减数分裂中期II，并接受了卵胞浆内单精子注射。所产生的胚胎在发育的第二天进行移植，成功妊娠并产下一名健康的活婴。剥除后体外成熟可被视为一种改善特定患者（如反应不良者）生殖结局的手段。
