Leoncini P, Cintorino M, Vindigni C, Leoncini L, Armellini D, Bugnoli M, Skalli O, Gabbiani G
Sclavo Research Center, Siena, Italy.
Virchows Arch A Pathol Anat Histopathol. 1988;412(4):329-37. doi: 10.1007/BF00750259.
We have evaluated by means of immunocytochemistry the distribution of various cytoskeletal and contractile proteins (cytokeratins, vimentin, desmin and alpha-smooth muscle actin) in 23 salivary or lacrimal gland primary tumours (15 pleomorphic adenomas and 8 carcinomas in pleomorphic adenoma), one third of which contained areas of normal gland. Normal epithelial luminal cells were stained by cytokeratin antibodies with a general specificity, while myoepithelial cells were selectively stained by a monoclonal antibody (SK2-27) reacting in immunoblots with cytokeratin polypeptides 14, 16 and 17, according to the classification of Moll et al. (1982) and by an antibody directed against alpha-smooth muscle actin (Skalli et al. 1986). In pleomorphic adenomas, both epithelial and myoepithelial cells displayed typical topographic distributions; moreover, myoepithelial cells showed two distinct cytoskeletal phenotypes. These findings could account in part for the heterogeneity of aspects observed in this tumour. In carcinomas, malignant cells were always positive to cytokeratin antibodies with general specificity and myoepithelial cells were absent as judged by anticytokeratin SK2-27 and anti-alpha-smooth muscle actin immunostainings. However, interestingly, there was in all cases a strong positivity for alpha-smooth muscle actin in stromal cells, similarly to what has previously been described for mammary carcinoma (Skalli et al. 1986). Our findings may be useful for the interpretation of the histogenesis of salivary and lacrimal tumour and stromal cells.
我们通过免疫细胞化学方法评估了23例唾液腺或泪腺原发性肿瘤（15例多形性腺瘤和8例多形性腺瘤中的癌）中各种细胞骨架和收缩蛋白（细胞角蛋白、波形蛋白、结蛋白和α-平滑肌肌动蛋白）的分布，其中三分之一含有正常腺体区域。正常上皮腔面细胞被具有一般特异性的细胞角蛋白抗体染色，而肌上皮细胞则被一种单克隆抗体（SK2-27）选择性染色，该抗体在免疫印迹中与细胞角蛋白多肽14、16和17反应，根据Moll等人（1982年）的分类，以及被一种针对α-平滑肌肌动蛋白的抗体（Skalli等人，1986年）染色。在多形性腺瘤中，上皮细胞和肌上皮细胞均显示出典型的拓扑分布；此外，肌上皮细胞表现出两种不同的细胞骨架表型。这些发现可以部分解释该肿瘤中观察到的外观异质性。在癌中，恶性细胞总是对具有一般特异性的细胞角蛋白抗体呈阳性，通过抗细胞角蛋白SK2-27和抗α-平滑肌肌动蛋白免疫染色判断，肌上皮细胞缺失。然而，有趣的是，在所有病例中，基质细胞中的α-平滑肌肌动蛋白均呈强阳性，这与先前对乳腺癌的描述类似（Skalli等人，1986年）。我们的发现可能有助于解释唾液腺和泪腺肿瘤及基质细胞的组织发生。
