Kasper M, Stosiek P
Department of Pathology, District Hospital Görlitz, German Democratic Republic.
Cell Tissue Res. 1989 Sep;257(3):661-4. doi: 10.1007/BF00221479.
The anatomical distribution of cytokeratins and vimentin was investigated by means of immunohistochemistry in the human epididymis. Epithelial cells of the ductuli efferentes and the corpus epididymidis were positive for cytokeratins and vimentin. The expression of epithelial vimentin decreased toward the cauda epididymidis, whereas cytokeratins remained unchanged. The epithelium of the ductus deferens was negative when antibodies against vimentin were used. With monoclonal antibodies to individual cytokeratins, the presence of cytokeratins 7, 8, 18, and 19 was demonstrated histochemically throughout the epithelium of the epididymis. Monoclonal antibodies specific for cytokeratin 17 allowed immunohistochemical differentiation between the ductuli efferentes and the ductus epididymidis.
通过免疫组织化学方法研究了细胞角蛋白和波形蛋白在人类附睾中的解剖分布。输出小管和附睾体的上皮细胞对细胞角蛋白和波形蛋白呈阳性反应。上皮波形蛋白的表达向附睾尾部逐渐降低，而细胞角蛋白保持不变。当使用抗波形蛋白抗体时，输精管上皮呈阴性。利用针对单个细胞角蛋白的单克隆抗体，通过组织化学方法证实了细胞角蛋白7、8、18和19在附睾上皮中均有表达。针对细胞角蛋白17的单克隆抗体能够通过免疫组织化学方法区分输出小管和附睾管。
