Hay D I, Moreno E C
J Dent Res. 1979 Mar;58(Spec Issue B):930-42. doi: 10.1177/00220345790580024701.
Studies are reviewed which identify the interacting groups involved in protein adsorption onto hydroxyapatite. Thus, carboxyl and phosphoester groups bind to calcium sites in the adsorbent, and basic groups bind to phosphate sites. Detailed adsorption studies have been performed to quantitate some of these interactions. An adsorption model, based on the Langmuir adsorption isotherm, adequately fitted the data from experiments using selected amino acids, bovine albumin and two human salivary proteins. Adsorption parameters (affinities and maximum number of sites) were obtained for several apatitic adsorbents, with affinities increasing considerably in the series hydroxy- (HA), fluorhydroxy- (FHA) and fluorapatite (FA). A modest increase in the number of sites was also noted. The change in adsorption behavior, with increasing fluoride content, was attributed to a reduction in the surface free energy of the adsorbent, with a concomitant decrease in the interaction of the adsorbent with water, and a consequent enhancement of the adsorption bond. It is suggested that this effect may play a role in the cariostatic effect of fluoride. Unusual structural aspects of the salivary proteins are discussed in relation to their adsorption behavior, and the molecular segments responsible for binding to the adsorbent tentatively identified.
本文综述了一些研究，这些研究确定了参与蛋白质吸附到羟基磷灰石上的相互作用基团。因此，羧基和磷酸酯基团与吸附剂中的钙位点结合，而碱性基团与磷酸盐位点结合。已经进行了详细的吸附研究以定量其中一些相互作用。基于朗缪尔吸附等温线的吸附模型很好地拟合了使用选定氨基酸、牛血清白蛋白和两种人类唾液蛋白的实验数据。获得了几种磷灰石吸附剂的吸附参数（亲和力和最大结合位点数量），在羟基磷灰石（HA）、氟羟基磷灰石（FHA）和氟磷灰石（FA）系列中，亲和力显著增加。还注意到结合位点数量有适度增加。随着氟含量增加，吸附行为的变化归因于吸附剂表面自由能的降低，同时吸附剂与水的相互作用减少，从而增强了吸附键。有人认为这种效应可能在氟的防龋作用中发挥作用。讨论了唾液蛋白不同寻常的结构特征与其吸附行为的关系，并初步确定了负责与吸附剂结合的分子片段。
