Lamkin M S, Arancillo A A, Oppenheim F G
Department of Periodontology and Oral Biology, Boston University Medical Center, Massachusetts 02118, USA.
J Dent Res. 1996 Feb;75(2):803-8. doi: 10.1177/00220345960750021101.
Salivary proteins bind to enamel surfaces and hydroxyapatite in a highly selective manner. Numerous studies have identified these proteins as primarily proline-rich proteins, cystatins, statherin, and histatins. Previously, the hydroxyapatite-binding potential of these proteins had been characterized in systems consisting of singly purified protein and adsorbent. The purpose of this study was to investigate the adsorption of each protein in the presence of complete salivary secretion. Proteins, shown to adsorb to hydroxyapatite, were purified, biotinylated, and added back to the remaining proteins to form a series of reconstituted secretions. The adsorption of each biotinylated protein in the reconstituted secretion to hydroxyapatite was then measured as a function of time. Results indicated that three different adsorption patterns occur. A simple hyperbolic pattern is characteristic of amylase, glycosylated proline-rich protein (PRG), and cystatin. A faster adsorption process is observed for PRP-3, PRP-4, PIF-f, and statherin. A more complex pattern, exhibiting a rapid phase followed by a slower phase, is characteristic of PRP-1, PRP-2, PIF-s, and histatins. These results suggest that there are different adsorption processes involved in the binding of salivary proteins to hydroxyapatite. Two possible mechanisms are direct adsorption of protein to hydroxyapatite and indirect adsorption of protein by interacting with other proteins already bound to hydroxyapatite.
唾液蛋白以高度选择性的方式与牙釉质表面和羟基磷灰石结合。大量研究已确定这些蛋白主要是富含脯氨酸的蛋白、胱抑素、富组蛋白和磷蛋白。此前，这些蛋白与羟基磷灰石的结合潜力已在由单一纯化蛋白和吸附剂组成的系统中得到表征。本研究的目的是调查在完整唾液分泌存在的情况下每种蛋白的吸附情况。将已证明能吸附到羟基磷灰石上的蛋白进行纯化、生物素化，然后添加回其余蛋白中以形成一系列重组分泌物。然后测量重组分泌物中每种生物素化蛋白对羟基磷灰石的吸附随时间的变化情况。结果表明出现了三种不同的吸附模式。简单的双曲线模式是淀粉酶、糖基化富含脯氨酸蛋白（PRG）和胱抑素的特征。对于PRP - 3、PRP - 4、PIF - f和磷蛋白，观察到更快的吸附过程。一种更复杂的模式，先出现快速阶段，随后是较慢阶段，是PRP - 1、PRP - 2、PIF - s和富组蛋白的特征。这些结果表明，唾液蛋白与羟基磷灰石结合涉及不同的吸附过程。两种可能的机制是蛋白直接吸附到羟基磷灰石上以及蛋白通过与已结合到羟基磷灰石上的其他蛋白相互作用而间接吸附。
