Chrispens J, Weliky B, Bringas P, Slavkin H
J Dent Res. 1979 Mar;58(Spec Issue B):988-90. doi: 10.1177/00220345790580025401.
Our laboratory is interested in determining the number of enamel protein gene products which characterize the ameloblast phenotype, and to what extent these proteins are degraded as a function of enamel maturation. Many investigators have reported a large number of heterogeneous proteins within the enamel matrix (Eggert, Allen and Burgess, 1973; Weidmann and Eyre, 1971). One explanation for this apparent heterogeneity may be the uncertain age of the analyzed enamel matrix; specimens may have undergone degradative processes associated with enamel maturation. To overcome this difficulty, our laboratory selected 26-day embryonic New Zealand White (NZW) rabbit incisor and molar tooth organs to isolate enamel protein(s). At this developmental stage it is possible to identify newly-secreted enamel matrix proteins.
我们的实验室致力于确定表征成釉细胞表型的釉质蛋白基因产物的数量，以及这些蛋白质在釉质成熟过程中降解的程度。许多研究人员报告说，釉质基质中存在大量异质蛋白质（Eggert、Allen和Burgess，1973年；Weidmann和Eyre，1971年）。这种明显异质性的一个解释可能是所分析的釉质基质的年龄不确定；标本可能经历了与釉质成熟相关的降解过程。为了克服这一困难，我们的实验室选择了26天胚胎期的新西兰白兔（NZW）的切牙和磨牙器官来分离釉质蛋白。在这个发育阶段，可以识别新分泌的釉质基质蛋白。
