Hyatt-Fischer H, Chrispens J, O'Keefe D, Slavkin H C
J Dent Res. 1979 Mar;58(Spec Issue B):1008-9. doi: 10.1177/002203457905800205011.
Embryonic mammalian enamel extracellular matrix is immunogenic. Antisera has been produced in New Zealand white rabbits using 5-day-old (post-natal) C57B1/6J mandibular and maxillary incisor and molar tooth organs as immunogens. The expression of secretory amelogenesis in mouse molar tooth organs was studied from the "cap stage" (circa 17-day fetus) to the fifth day of postnatal odontogenesis using indirect immunofluorescent microscopy. The specificity of the antisera for enamel matrix secretion was unequivocal. Secretory amelogenesis was observed in molar tooth organs as early as day-2 postnatal age. These reagents and methods provide a significant strategy in studies of epithelial-mesenchymal interactions during tooth development.
胚胎期哺乳动物的牙釉质细胞外基质具有免疫原性。已使用5日龄（出生后）C57B1/6J小鼠的下颌和上颌切牙及磨牙牙器官作为免疫原，在新西兰白兔中制备了抗血清。利用间接免疫荧光显微镜，研究了小鼠磨牙牙器官从“帽状期”（约17日龄胎儿）到出生后牙发生第5天分泌期釉质形成的表达情况。抗血清对牙釉质基质分泌的特异性是明确的。在出生后第2天的磨牙牙器官中就观察到了分泌期釉质形成。这些试剂和方法为研究牙齿发育过程中的上皮-间充质相互作用提供了重要策略。
