Faustino-Rocha Ana I, Gama Adelina, Neuparth Maria J, Oliveira Paula A, Ferreira Rita, Ginja Mário
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.
Anticancer Res. 2017 Mar;37(3):1013-1021. doi: 10.21873/anticanres.11411.
BACKGROUND/AIM: The effects of mast cells on carcinogenesis is not yet fully understood. This work aimed to disclose the role of mast cells in mammary carcinogenesis in a rat model.
Mammary tumors were induced by the administration of N-methyl-N-nitrosourea (MNU) in three groups of rats. Animals from one group were treated with ketotifen immediately after MNU administration, and animals from another only received ketotifen after the development of the first mammary tumor. The biochemical profile was determined. Mammary tumors were evaluated by histopathology and immunohistochemistry.
Animals from ketotifen-treated groups developed fewer mammary tumors, higher number of mammary lesions and had lower histamine levels when compared to non-treated animals. Animals treated with ketotifen immediately after MNU exhibited the lowest proliferative and apoptotic indexes.
The mainly positive effect of the inhibition of mast cell degranulation seems to be the reduction of tumor proliferation when the mast cell degranulation was inhibited before tumor development.
背景/目的：肥大细胞在致癌过程中的作用尚未完全明确。本研究旨在揭示肥大细胞在大鼠乳腺癌发生过程中的作用。
给三组大鼠注射N-甲基-N-亚硝基脲（MNU）诱导乳腺肿瘤。一组动物在注射MNU后立即用酮替芬治疗，另一组动物仅在第一个乳腺肿瘤出现后才接受酮替芬治疗。测定生化指标。通过组织病理学和免疫组织化学评估乳腺肿瘤。
与未治疗的动物相比，接受酮替芬治疗组的动物发生的乳腺肿瘤较少，乳腺病变数量较多，组胺水平较低。在注射MNU后立即用酮替芬治疗的动物增殖和凋亡指数最低。
当在肿瘤发生前抑制肥大细胞脱颗粒时，抑制肥大细胞脱颗粒的主要积极作用似乎是减少肿瘤增殖。
