Chen Huan, Jie Chong, Tang Lu-Ping, Meng He, Li Xiao-Bo, Li Yan-Bing, Chen Le-Xing, Yan Chang, Kurihara Hiroshi, Li Yi-Fang, He Rong-Rong
Anti-Stress and Health Research Center, College of Pharmacy, Jinan University, Guangzhou 510632, China.
Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China.
Phytomedicine. 2017 Apr 15;27:52-62. doi: 10.1016/j.phymed.2017.02.004. Epub 2017 Feb 14.
KangBingDu (KBD) is a classic traditional Chinese medicinal formula widely used to treat influenza. However, little information is available from controlled studies regarding the anti-influenza pharmacological activities of KBD and its underlying mechanisms, at least partly due to the lack of appropriate study models.
We hypothesized that KBD might provide a protection against influenza infection by reducing the host's susceptibility to viruses. To prove it, mouse restraint stress model was employed.
Mice were restricted and infected with influenza virus. KBD (13 and 26mg/kg/d) was orally administrated to mice from the first day of restraint stress and lasted for 7 days (twice a day). Mice were monitored daily for morbidity, symptom severity, and mortality for 21 days. The histopathologic changes were examined. For the study of mechanisms of action, we investigated whether KBD could promote mitochondria antiviral signaling protein (MAVS)-mediated antiviral signal and inhibit nuclear factor-kappa B (NF-κB)-mediated inflammation response.
KBD significantly decreased the susceptibility of restraint mice to influenza virus, as evidenced by lowered mortality, attenuated inflammation and reduced viral replications in lungs. Further results revealed that KBD elevated the protein expression of MAVS, which subsequently increased the IFN-β and IFITM3 protein levels, thereby helping to fight viral infections. Finally, we identified that (R,S)-goitrin, mangiferin, forsythin and forsythoside A were effective components in KBD against influenza viral infections.
KBD can reduce the susceptibility to influenza virus via mitochondrial antiviral signaling.
抗病毒（KBD）是一种广泛用于治疗流感的经典中药配方。然而，关于KBD的抗流感药理活性及其潜在机制的对照研究信息很少，至少部分原因是缺乏合适的研究模型。
我们假设KBD可能通过降低宿主对病毒的易感性来提供对流感感染的保护。为了证明这一点，采用了小鼠束缚应激模型。
将小鼠束缚并感染流感病毒。从束缚应激的第一天开始，每天两次给小鼠口服KBD（13和26mg/kg/d），持续7天。每天监测小鼠21天的发病率、症状严重程度和死亡率。检查组织病理学变化。为了研究作用机制，我们研究了KBD是否能促进线粒体抗病毒信号蛋白（MAVS）介导的抗病毒信号，并抑制核因子-κB（NF-κB）介导的炎症反应。
KBD显著降低了束缚小鼠对流感病毒的易感性，表现为死亡率降低、炎症减轻和肺内病毒复制减少。进一步的结果表明，KBD提高了MAVS的蛋白表达，随后增加了IFN-β和IFITM3蛋白水平，从而有助于对抗病毒感染。最后，我们确定（R，S）-甲状腺素、芒果苷、连翘苷和连翘酯苷A是KBD中抗流感病毒感染的有效成分。
KBD可通过线粒体抗病毒信号降低对流感病毒的易感性。
