College of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and Formulas), Key Laboratory of Beijing (Syndromes and Formulas), Beijing University of Chinese Medicine, Beijing, China; Institute of Basic Theory for Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, China.
College of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
J Ethnopharmacol. 2017 Apr 18;202:162-171. doi: 10.1016/j.jep.2017.03.015. Epub 2017 Mar 16.
Euonymus alatus, Radix trichosanthis, Panax notoginseng and Coptis chinensis are popular plants used in traditional Chinese medicine to treat diabetes.
The aim of the study is to investigate the therapeutic effect of the active components of Euonymus alatus, Radix trichosanthis, Panax notoginseng and Coptis chinensis (cERPC) on diabetic peripheral neuropathy in the rats and explore the underlying mechanism involved.
After diabetes was induced in rats for 20 weeks, cERPC or water was administered for 12 weeks. After a hot plate test, motor nerve conduction velocity and sciatic nerve blood flow were determined; the sciatic nerves were isolated for toluidine blue staining; and the fibre area, fibre diameter, axon area, axon diameter and myelin thickness were evaluated. The levels of the myelin basic protein, myelin protein zero, Oct6 and Krox20 were measured by western blot or immunofluorescence.
cERPC was efficient in reducing the response latency, increasing motor nerve conduction velocity, enhancing sciatic nerve blood flow and ameliorating the pathological changes in diabetic rats. cERPC also had a role in increasing the levels of myelin basic protein and myelin protein zero and improving the expression of Oct6 and Krox20 in sciatic nerves of diabetic rats.
cERPC ameliorates diabetic peripheral neuropathy by attenuating electrophysiological, circulatory and morphological alterations, which is mediated by the Oct6-Krox20 pathway.
卫矛、瓜蒌根、三七和黄连是传统中药中用于治疗糖尿病的常用植物。
本研究旨在探讨卫矛、瓜蒌根、三七和黄连（cERPC）活性成分对糖尿病周围神经病变大鼠的治疗作用，并探讨其潜在机制。
糖尿病大鼠诱导 20 周后，给予 cERPC 或水治疗 12 周。热板试验后，测定运动神经传导速度和坐骨神经血流量；分离坐骨神经进行甲苯胺蓝染色；评估纤维面积、纤维直径、轴突面积、轴突直径和髓鞘厚度。采用 Western blot 或免疫荧光法测定髓鞘碱性蛋白、髓鞘蛋白零、Oct6 和 Krox20 的水平。
cERPC 有效降低了反应潜伏期，增加了运动神经传导速度，增强了坐骨神经血流量，并改善了糖尿病大鼠的病理变化。cERPC 还能增加髓鞘碱性蛋白和髓鞘蛋白零的水平，并改善糖尿病大鼠坐骨神经中 Oct6 和 Krox20 的表达。
cERPC 通过减轻电生理、循环和形态改变来改善糖尿病周围神经病变，其作用机制是通过 Oct6-Krox20 通路介导的。
