Üstün Ramazan, Oğuz Elif Kaval, Şeker Ayşe, Taspinar Filiz
Neuroscience Research Unit, School of Medicine, Van Yüzüncü Yıl University, Van 65080, Turkey.
Department of Physiology, School of Medicine, Van Yüzüncü Yıl University, Van 65080, Turkey.
Exp Ther Med. 2024 Jul 2;28(3):345. doi: 10.3892/etm.2024.12634. eCollection 2024 Sep.
Traumatic and postoperative hemorrhages are life-threatening complications. Ankaferd BloodStopper (ABS) is a potent topical hemostatic agent to stop bleeding. However, ABS is associated with nerve toxicity. The present study aimed to investigate the functional and structural neurodegenerative effects of ABS in a mouse model. A total of 30 male BALB/c mice, aged 6-8 weeks, were randomly divided into control group (no treatment), a sham group (treated with saline) and an experimental group (treated with ABS). In the saline and the ABS groups, the right sciatic nerve was surgically exposed and treated with saline or ABS, respectively. No surgical procedure was performed in the control group. On day 7 post-treatment, functional changes of the sciatic nerve were evaluated by a horizontal ladder rung walking task. Structural changes were assessed with immunohistochemistry. In the horizontal ladder rung walking test, the gait impairment was proportional to the severity of sciatic nerve damage, with the ABS group showing a significantly higher rate of errors than the control and saline groups. Immunohistochemistry demonstrated extensive degeneration and deformation in the axons and myelin sheath of the sciatic nerve in the ABS group. The results provide compelling evidence for the neurotoxicity of ABS.
创伤性出血和术后出血是危及生命的并发症。安卡非德止血剂（ABS）是一种有效的局部止血剂。然而，ABS与神经毒性有关。本研究旨在探讨ABS在小鼠模型中的功能和结构神经退行性影响。总共30只6 - 8周龄的雄性BALB/c小鼠被随机分为对照组（未治疗）、假手术组（用生理盐水治疗）和实验组（用ABS治疗）。在生理盐水组和ABS组中，手术暴露右侧坐骨神经，分别用生理盐水或ABS进行处理。对照组未进行手术操作。治疗后第7天，通过水平梯级行走任务评估坐骨神经的功能变化。用免疫组织化学评估结构变化。在水平梯级行走试验中，步态损伤与坐骨神经损伤的严重程度成正比，ABS组的错误率明显高于对照组和生理盐水组。免疫组织化学显示ABS组坐骨神经的轴突和髓鞘广泛变性和变形。结果为ABS的神经毒性提供了有力证据。
