Khodayary Ramin, Nikokar Iraj, Mobayen Mohammad Reza, Afrasiabi Farhad, Araghian Afshin, Elmi Ali, Moradzadeh Meisam
Department of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Medical Biotechnology Research Center, School of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran.
BMC Res Notes. 2019 Jan 15;12(1):28. doi: 10.1186/s13104-019-4071-0.
The present study aimed to determine the prevalence of virulence factors and antimicrobial resistance profile of Pseudomonas aeruginosa strains isolated from Iranian burn patients.
This cross-sectional study performed on 100 P. aeruginosa isolates which were recovered from burn wound specimens in 2014-2015. All presumptive isolates were identified by standard microbiologic tests. Antimicrobial susceptibility test was carried out by disk diffusion method. The presence of virulence genes was determined by PCR method. Antibiotic susceptibility results revealed that the isolates were mostly susceptible to amikacin (61%), ceftazidime (60%), and imipenem (55%). Moreover, 59% of the isolates were multi-drug resistance (MDR). The most prevalent MDR pattern was aminoglycosides-penicillins-fluoroquinolones-carbapenems (15%). The presence of exoT, exoY, exoS and exoU genes was detected in 100%, 100%, 59%, and 41% of the tested isolates, respectively. Results points out the pattern of MDR and genetic diversity of type III secretion system among P. aeruginosa strains isolated from the burn population. Overall, the association of MDR and the presence of the specific virulence genes can be a predictive marker for the persistence of these isolates in the hospitals and subsequently a worse clinical condition for the affected patients.
本研究旨在确定从伊朗烧伤患者中分离出的铜绿假单胞菌菌株的毒力因子流行情况及抗菌药物耐药谱。
本横断面研究对2014 - 2015年从烧伤创面标本中分离出的100株铜绿假单胞菌进行。所有疑似分离株均通过标准微生物学试验鉴定。采用纸片扩散法进行抗菌药物敏感性试验。通过PCR法确定毒力基因的存在情况。抗生素敏感性结果显示，分离株大多对阿米卡星（61%）、头孢他啶（60%）和亚胺培南（55%）敏感。此外，59%的分离株为多重耐药（MDR）。最常见的MDR模式是氨基糖苷类 - 青霉素类 - 氟喹诺酮类 - 碳青霉烯类（15%）。在分别100%、100%、59%和41%的受试分离株中检测到exoT、exoY、exoS和exoU基因的存在。结果指出了从烧伤人群中分离出的铜绿假单胞菌菌株的MDR模式和III型分泌系统的遗传多样性。总体而言，MDR与特定毒力基因的存在之间的关联可能是这些分离株在医院持续存在以及随后受影响患者临床状况更差的预测标志物。
