Biodegradation of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) by Phanerochaete chrysosporium in the presence of Cd.

Aerobic biodegradation of 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) by Phanerochaete chrysosporium in the presence of Cd was investigated in this study. The results showed that P. chrysosporium could effectively degrade BDE-47, and its extracellular enzyme played an important role in the process of decomposition. BDE-47 biodegradation by fungi was more tolerant than extracellular enzyme in the presence of Cd. Also, both of the activity of two typical enzymes, MnP and LiP, descended with ascended Cd concentration. Based on the four mono-hydroxylated PBDEs (5-OH-BDE-47, 4'-OH-BDE-17, 6-OH-BDE-47, and 2'-OH-BDE-28) and two bromophenols (2,4-DBP, 4-BP) detected, three possible degradation pathways were proposed, inferring that BDE-47 was more easily to transform via hydroxylation. With addition of Cd, the types of degradation products did not change, merely a variation of the content of these products observed. Meanwhile, the major metabolites of BDE-47, bromophenol compounds, have been found to be transformed or even mineralized by P. chrysosporium quickly, which also helped better explain why the amounts of BDE-47 decomposed did not match with that of the metabolites detected.