Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China; Consortium on Environment, Health, Education and Research (CHEER), and Department of Science and Environmental Studies, The Education University of Hong Kong, Tai Po, Hong Kong SAR, PR China.
Sci Total Environ. 2018 Sep 1;634:417-426. doi: 10.1016/j.scitotenv.2018.03.310. Epub 2018 Apr 6.
A pot experiment was conducted to investigate the effect of a series of β-cyclodextrin (β-CD) concentrations on bioremediation of soil co-contaminated with Cd and BDE-209 using amaranth and the white-rot fungus Phanerochaete chrysosporium, with BDE-209 degrading ability. Results showed that the white-rot fungus was beneficial to the growth of amaranth, Cd uptake and BDE-209 degradation. Addition of β-CD further increased biomass of both shoots and roots, shoot Cd concentrations and contents, chlorophyll concentrations and soil manganese peroxidase (MnP) activities. Furthermore, well-organized mesophyll cells were observed in β-CD treatments, implying that the combination of white-rot fungus and β-CD can alleviate the stresses of Cd and BDE-209 to mesophyll cells. The BDE-209 degradation rate was positively correlated to β-CD concentration and MnP activity in soil. Our results also revealed that RF+β treatment possessed the greatest Cd removal efficiency due to its well-configured mesophyll cells and the highest shoot biomass, chlorophyll concentration, and shoot Cd concentration. Considering simultaneous removal of Cd and BDE-209 from soil, using 0.8% β-CD to amaranth inoculated with white-rot fungus is a promising way forward for the phytoremediation of soil co-contaminated with Cd and BDE-209. A high percentage of mono-BDE was detected in inoculated amaranth, suggesting that BDE-209 was debrominated into low brominated PBDEs by the fungus in soil, which were then absorbed and further debrominated into mono-BDE in the plant.
采用盆栽实验研究了一系列β-环糊精（β-CD）浓度对添加具有 BDE-209 降解能力的白腐真菌糙皮侧耳（Phanerochaete chrysosporium）后受 Cd 和 BDE-209 复合污染土壤的生物修复效果。结果表明，白腐真菌有利于苋菜生长、Cd 吸收和 BDE-209 降解。添加β-CD 进一步增加了地上部和地下部生物量、地上部 Cd 浓度和含量、叶绿素浓度和土壤锰过氧化物酶（MnP）活性。此外，β-CD 处理中观察到组织排列整齐的叶肉细胞，表明白腐真菌和β-CD 的结合可以减轻 Cd 和 BDE-209 对叶肉细胞的胁迫。BDE-209 的降解率与土壤中β-CD 浓度和 MnP 活性呈正相关。本研究还表明，由于具有良好的叶肉细胞结构和较高的地上部生物量、叶绿素浓度和地上部 Cd 浓度，RF+β 处理具有最大的 Cd 去除效率。考虑到同时去除土壤中的 Cd 和 BDE-209，向接种白腐真菌的苋菜中添加 0.8%β-CD 是一种很有前景的方法，可用于修复同时受到 Cd 和 BDE-209 污染的土壤。接种苋菜中检测到高比例的单溴化二苯醚（mono-BDE），表明 BDE-209 在土壤中被真菌脱溴化为低溴化 PBDEs，然后被植物吸收并进一步脱溴化为 mono-BDE。
