Cardiovascular Innovation Institute, Department of Physiology, University of Louisville School of Medicine , Louisville, Kentucky.
Tissue Eng Part B Rev. 2018 Aug;24(4):289-299. doi: 10.1089/ten.TEB.2017.0061. Epub 2017 Apr 13.
A major challenge in tissue engineering is the generation of sufficient volumes of viable tissue for organ transplant. The development of a stable, mature vasculature is required to sustain the metabolic and functional activities of engineered tissues. Adipose stromal vascular fraction (SVF) cells are an easily accessible, heterogeneous cell system comprised of endothelial cells, macrophages, pericytes, and various stem cell populations. Collectively, SVF has been shown to spontaneously form vessel-like networks in vitro and robust, patent, and functional vasculatures in vivo. Capitalizing on this ability, we and others have demonstrated adipose SVF's utility in generating and augmenting engineered liver, cardiac, and vascular tissues, to name a few. This review highlights the scientific origins of SVF, the use of SVF as a clinically relevant vascular source, various SVF constituents and their roles, and practical considerations associated with isolating SVF for various tissue engineering applications.
组织工程面临的一个主要挑战是为器官移植生成足够体积的有活力的组织。需要稳定的、成熟的脉管系统来维持工程组织的代谢和功能活动。脂肪基质血管部分 (SVF) 细胞是一种易于获取的异质细胞系统，由内皮细胞、巨噬细胞、周细胞和各种干细胞群体组成。SVF 已被证明可以在体外自发形成类似血管的网络，并在体内形成强健、通畅和功能正常的脉管系统。利用这一能力，我们和其他人已经证明了脂肪 SVF 在生成和增强工程肝脏、心脏和血管组织方面的效用，仅举几例。这篇综述重点介绍了 SVF 的科学起源、SVF 作为临床相关血管来源的用途、SVF 的各种成分及其作用，以及与各种组织工程应用中分离 SVF 相关的实际考虑因素。
