Brar A P S, Sood N K, Singla L D, Kaur P, Gupta K, Sandhu B S
Departments of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India.
Department of Teaching Veterinary Clinical Complex, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India.
J Parasit Dis. 2017 Mar;41(1):260-262. doi: 10.1007/s12639-016-0788-z. Epub 2016 Jun 22.
Cryptosporidiosis is an emerging waterborne protozoan disease and one of the major causes of neonatal diarrhea in humans and animals. But the disease remains under diagnosed due to lack of availability of special stains in majority of laboratories at primary health centers. Therefore, it requires a rapid screening test for routine diagnosis in conventional laboratory set up. In this pursuit, the present study was planned. During this study, fecal samples from 100 representative animals randomly selected from 17 out breaks of bovine calf diarrhea, were stained with modified Ziehl Neelsen staining (mZN) and Leishman's stain to demonstrate cryptosporidial oocysts and for routine fecal examination, respectively. By mZN staining, 25 cases confirmed the presence of cryptosporidial oocysts. However, examination of Leishman's stained fecal smears revealed round hollow unstained bodies resembling cryptosporidia in 20 cases. Therefore, a comparative morphometric analysis was made between the two techniques to determine their relative efficacy in demonstrating cryptosporidia in the feces of affected animals. The analyses showed that the Leishman's stain can be effective in making a presumptive diagnosis of cryptosporidiosis with a little experience. Confirmation of cryptosporidiosis was done by histopathological examination of intestinal sections of calves died during these out breaks. The findings appear to have great clinical value for routine laboratory screening of fecal samples for cryptosporidiosis as conventional Romanowsky stains are readily available and used for multipurpose examination in most of the laboratories at grass root level. Perusal of literature proved this to be the first attempt at easy diagnostics for cryptosporidiosis.
隐孢子虫病是一种新出现的经水传播的原生动物疾病，是人和动物新生儿腹泻的主要病因之一。但由于大多数基层卫生中心实验室缺乏特殊染色剂，该疾病仍未得到充分诊断。因此，在常规实验室环境中进行常规诊断需要一种快速筛查试验。为此，开展了本研究。在本研究中，从17起牛犊腹泻疫情中随机选取100只代表性动物的粪便样本，分别用改良齐-尼氏染色法（mZN）和利什曼染色法染色，以检测隐孢子虫卵囊和进行常规粪便检查。通过mZN染色，25例确诊存在隐孢子虫卵囊。然而，利什曼染色的粪便涂片检查显示，20例中有类似隐孢子虫的圆形中空未染色体。因此，对这两种技术进行了比较形态计量分析，以确定它们在检测患病动物粪便中隐孢子虫方面的相对效果。分析表明，利什曼染色法在有一定经验的情况下，可有效进行隐孢子虫病的初步诊断。通过对这些疫情期间死亡犊牛肠道切片的组织病理学检查确诊隐孢子虫病。这些发现对于基层实验室常规筛查粪便样本中的隐孢子虫病似乎具有重要的临床价值，因为传统的罗曼诺夫斯基染色剂很容易获得，并且在大多数基层实验室中用于多种用途的检查。查阅文献证明，这是首次尝试对隐孢子虫病进行简易诊断。
