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本文引用的文献

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Flow cytometric detection of Cryptosporidium oocysts in human stool samples.流式细胞术检测人粪便样本中的隐孢子虫卵囊
J Clin Microbiol. 1997 Aug;35(8):2013-7. doi: 10.1128/jcm.35.8.2013-2017.1997.
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Epidemiologic aspects of human cryptosporidiosis and the role of waterborne transmission.人类隐孢子虫病的流行病学特征及经水传播的作用。
Epidemiol Rev. 1996;18(2):118-36. doi: 10.1093/oxfordjournals.epirev.a017920.
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Surveillance for waterborne-disease outbreaks--United States, 1993-1994.美国1993 - 1994年水源性疾病暴发监测
MMWR CDC Surveill Summ. 1996 Apr 12;45(1):1-33.
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Review of equine Cryptosporidium infection.马隐孢子虫感染综述。
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A new method for evaluating experimental cryptosporidial parasite loads using immunofluorescent flow cytometry.一种使用免疫荧光流式细胞术评估实验性隐孢子虫寄生虫负荷的新方法。
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6
Large community outbreak of cryptosporidiosis due to contamination of a filtered public water supply.因过滤后的公共供水系统受污染导致隐孢子虫病在社区大规模爆发。
N Engl J Med. 1989 May 25;320(21):1372-6. doi: 10.1056/NEJM198905253202103.
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Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection.用于隐孢子虫卵囊检测的传统染色方法与基于单克隆抗体的方法的比较。
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8
Threshold of detection of Cryptosporidium oocysts in human stool specimens: evidence for low sensitivity of current diagnostic methods.人类粪便标本中隐孢子虫卵囊的检测阈值：当前诊断方法低敏感性的证据
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马体内微小隐孢子虫的检测：抗酸染色、免疫荧光测定法及流式细胞术的阈值

Detection of Cryptosporidium parvum in horses: thresholds of acid-fast stain, immunofluorescence assay, and flow cytometry.

作者信息

Cole D J, Snowden K, Cohen N D, Smith R

机构信息

Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4475, USA.

出版信息

J Clin Microbiol. 1999 Feb;37(2):457-60. doi: 10.1128/JCM.37.2.457-460.1999.

DOI:10.1128/JCM.37.2.457-460.1999
PMID:9889243
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC84341/
Abstract

Feces collected from three asymptomatic horses and seeded with Cryptosporidium parvum oocysts (10(1) to 10(6)/g of feces) were evaluated by acid-fast staining (AF), an immunofluorescent antibody (IFA) technique, and flow cytometry. The thresholds of detection were 5 x 10(5) oocysts/g of feces for the IFA and AF techniques and 5 x 10(4) oocysts/g for flow cytometry.

摘要

收集了三头无症状马匹的粪便，并接种了微小隐孢子虫卵囊（每克粪便含10¹至10⁶个卵囊），采用抗酸染色（AF）、免疫荧光抗体（IFA）技术和流式细胞术进行评估。IFA和AF技术的检测阈值为每克粪便5×10⁵个卵囊，流式细胞术的检测阈值为每克粪便5×10⁴个卵囊。