马体内微小隐孢子虫的检测：抗酸染色、免疫荧光测定法及流式细胞术的阈值
Detection of Cryptosporidium parvum in horses: thresholds of acid-fast stain, immunofluorescence assay, and flow cytometry.
作者信息
Cole D J, Snowden K, Cohen N D, Smith R
机构信息
Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4475, USA.
出版信息
J Clin Microbiol. 1999 Feb;37(2):457-60. doi: 10.1128/JCM.37.2.457-460.1999.
Abstract
Feces collected from three asymptomatic horses and seeded with Cryptosporidium parvum oocysts (10(1) to 10(6)/g of feces) were evaluated by acid-fast staining (AF), an immunofluorescent antibody (IFA) technique, and flow cytometry. The thresholds of detection were 5 x 10(5) oocysts/g of feces for the IFA and AF techniques and 5 x 10(4) oocysts/g for flow cytometry.
摘要
收集了三头无症状马匹的粪便，并接种了微小隐孢子虫卵囊（每克粪便含10¹至10⁶个卵囊），采用抗酸染色（AF）、免疫荧光抗体（IFA）技术和流式细胞术进行评估。IFA和AF技术的检测阈值为每克粪便5×10⁵个卵囊，流式细胞术的检测阈值为每克粪便5×10⁴个卵囊。
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