Kavarizadeh Farzaneh, Khademvatan Shahram, Vazirianzadeh Babak, Feizhaddad Mohammad Hossein, Zarean Mehdi
Department of Medical Parasitology and Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Medical Parasitology and Mycology and Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.
J Parasit Dis. 2017 Mar;41(1):274-281. doi: 10.1007/s12639-016-0792-3. Epub 2016 Jun 14.
Cutaneous leishmaniasis (CL) is vector borne parasitic disease, considered as public health problem especially in border of Iran and Iraq, Dehloran County (Musian district). The aim of this study was molecular identification of parasites in sandfly as vectors of Leishmaniasis. Totally 280 female sandflies were trapped by sticky traps from 7 rural areas of Musiyan in September-November 2012. All sandflies were identified using morphological characters of the head and abdominal terminalia. DNA was extracted from female sandflies and was identified using PCR and sequencing. All 280 trapped sandflies were identified as and infections were detected in 3.2 % out of 280 female sandflies. All leishmania were identified as and submitted in Gene bank as: LC014642.1, LC014641.1, LC014640.1 and LC014639.1. Frequency of and infection with in studied regions showed that this vector is dominant in these areas.
皮肤利什曼病（CL）是一种媒介传播的寄生虫病，被视为一个公共卫生问题，特别是在伊朗与伊拉克边境的德赫洛兰县（穆西安区）。本研究的目的是对作为利什曼病传播媒介的白蛉体内的寄生虫进行分子鉴定。2012年9月至11月期间，用粘性诱捕器从穆西安的7个农村地区共捕获了280只雌性白蛉。所有白蛉均根据头部和腹部末端的形态特征进行鉴定。从雌性白蛉中提取DNA，并通过聚合酶链反应（PCR）和测序进行鉴定。所有280只捕获的白蛉均被鉴定出来，在280只雌性白蛉中有3.2%检测到感染。所有利什曼原虫均被鉴定出来，并以LC014642.1、LC014641.1、LC014640.1和LC014639.1的编号提交至基因库。研究区域内白蛉感染[具体寄生虫名称未明确写出]和[具体寄生虫名称未明确写出]的频率表明，这种传播媒介在这些地区占主导地位。
