Khoshnood Saleh, Tavalla Mehdi, Abtahi Seyed Mohammad, Jalali-Galousang Asadollah, Mohaghegh Mohammad-Ali, Khamesipour Faham, Hejazi Seyed Hossein
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Parasitology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Parasit Dis. 2021 Mar;45(1):263-272. doi: 10.1007/s12639-020-01298-2. Epub 2020 Nov 9.
Cutaneous leishmaniasis is a crucial vector-borne disease caused by various species of and is transmitted by several species of sandflies. The present study was conducted to describe sand fly fauna on vectors of leishmaniasis and performing molecular identification of isolates from them on the Iran-Iraq border. Entomological surveys were done from May to October 2016-2018 in 2 counties (Mehran and Dehloran) of Ilam province, west of Iran. Sandflies were collected by 40 Sticky Traps at each station. Samples were mounted for species identification using morphological characters of the head and abdominal terminalia. DNA was extracted from females, and isolates were identified through PCR on minicircle kDNA, followed by sequencing. A total of 5592 sandflies including 2 genera of and comprising 8 species of sand flies were detected. infection was detected in 3.33% of 300 tested female sandflies. was predominant in outdoor and indoor resting places. was determined as dominant vector of infection in Mehran and Dehloran counties, West of Iran. It seems the composition of sandfly species in the study area is almost similar to the other parts of Iran. A detailed description of the epidemiology and ecology of Phlebotomine sand flies needs to be established to accomplish effective vector control programs.
皮肤利什曼病是一种由多种利什曼原虫引起的重要媒介传播疾病，通过几种白蛉传播。本研究旨在描述利什曼病媒介上的白蛉种类，并对伊朗 - 伊拉克边境地区从这些白蛉分离出的利什曼原虫进行分子鉴定。2016年至2018年5月至10月期间，在伊朗西部伊拉姆省的两个县（梅赫兰和德洛兰）进行了昆虫学调查。每个站点用40个粘性诱捕器收集白蛉。利用头部和腹部末端的形态特征将样本制成标本进行种类鉴定。从雌性白蛉中提取DNA，并通过对微小环kDNA进行PCR扩增，随后进行测序来鉴定利什曼原虫分离株。共检测到5592只白蛉，包括白蛉属和司蛉属2个属，共8种白蛉。在300只受试雌性白蛉中，3.33%检测到利什曼原虫感染。在室外和室内栖息场所，长管白蛉占主导。长管白蛉被确定为伊朗西部梅赫兰和德洛兰县利什曼原虫感染的主要传播媒介。研究区域内白蛉种类的组成似乎与伊朗其他地区几乎相似。需要对白蛉的流行病学和生态学进行详细描述，以实施有效的媒介控制计划。
