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粘结前铸造桩的微生物学研究。

Microbiological Study of Cast Posts before Cementation.

作者信息

Vallejo-Labrada Maricela, Ojeda-Garces Juan Carlos

机构信息

Faculty of Dentistry, Universidad Cooperativa de Colombia, San Juan de Pasto, Nariño, Colombia.

出版信息

Int J Dent. 2017;2017:1090534. doi: 10.1155/2017/1090534. Epub 2017 Feb 20.

DOI:10.1155/2017/1090534
PMID:28316625
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5337860/
Abstract

This study identifies the most common microorganisms present in type III gold cast posts related to pulpal disease and evaluates the sterilization/disinfection method before cementation in the root canal. Forty-five type III gold cast posts were aseptically collected in sterile sealed plastic bags and taken to the microbiology laboratory to carry out the study: fifteen cast posts had no treatment, fifteen were disinfected (immersion in 70% alcohol during 15 minutes), and fifteen were autoclaved at 121°C for 15 minutes by using saturated steam under 15 psi pressure. By using a two-proportion -test, the difference was statistically significant ( > 0.05) and demonstrates that, in spite of the aseptic pattern used in the cast post collection and laboratory procedures, some cast posts arrive contaminated at the consulting office. The disinfection process worked out in a high percentage and demonstrated that the sterilization by autoclaving eliminated completely the pathogenic microbiota without affecting the cast post shape and integrity that could compromise their final fitting.

摘要

本研究确定了与牙髓疾病相关的III型金铸造桩中存在的最常见微生物,并评估了根管内粘固前的灭菌/消毒方法。45个III型金铸造桩被无菌收集在无菌密封塑料袋中,送至微生物实验室进行研究:15个铸造桩未作处理,15个进行了消毒(在70%酒精中浸泡15分钟),15个在15磅压力下使用饱和蒸汽于121°C高压灭菌15分钟。通过双比例检验,差异具有统计学意义(>0.05),表明尽管在铸造桩收集和实验室操作中采用了无菌模式,但一些铸造桩在咨询室时已被污染。消毒过程成功率很高,并且表明高压灭菌能完全消除致病微生物群,同时不影响铸造桩的形状和完整性,而这可能会损害其最终的适配性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/5337860/bef0147f1c3b/IJD2017-1090534.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/5337860/ba7301445345/IJD2017-1090534.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/5337860/274e0891fd09/IJD2017-1090534.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/5337860/bef0147f1c3b/IJD2017-1090534.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/5337860/ba7301445345/IJD2017-1090534.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/5337860/274e0891fd09/IJD2017-1090534.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5472/5337860/bef0147f1c3b/IJD2017-1090534.003.jpg

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1
Microbiological Study of Cast Posts before Cementation.粘结前铸造桩的微生物学研究。
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本文引用的文献

1
The Effect of Different Cleaning Protocols on Post Space: A SEM Study.不同清洁方案对桩腔的影响:一项扫描电子显微镜研究
Int J Dent. 2016;2016:1907124. doi: 10.1155/2016/1907124. Epub 2016 Sep 28.
2
Eight-Year Retrospective Study of the Critical Time Lapse between Root Canal Completion and Crown Placement: Its Influence on the Survival of Endodontically Treated Teeth.根管充填完成与冠修复之间关键时间间隔的八年回顾性研究:其对根管治疗后牙齿存留率的影响
J Endod. 2016 Nov;42(11):1598-1603. doi: 10.1016/j.joen.2016.08.006. Epub 2016 Sep 10.
3
In-vitro evaluation of coronal microbial leakage after post space tooth preparation.
Minerva Stomatol. 2016 Jun;65(3):127-33.
4
Evaluation of immediate and delayed post space preparation on sealing ability of Resilon-Epiphany and Gutta percha-AH plus sealer.根管预备后即刻及延迟对Resilon-Epiphany和牙胶尖-AH plus封闭剂封闭能力的评估。
J Conserv Dent. 2013 Nov;16(6):514-7. doi: 10.4103/0972-0707.120962.
5
Enterococcus faecalis and Candida albicans in the dental root canal and periapical infections.粪肠球菌和白色念珠菌与牙根根管及根尖周感染
Bratisl Lek Listy. 2013;114(12):716-20.
6
Coronal microleakage of endodontically treated teeth with intracanal post exposed to fresh human saliva.根管治疗后伴有管内桩外露的牙齿在新鲜人唾液中冠向微渗漏的研究
J Appl Oral Sci. 2013 Sep-Oct;21(5):403-8. doi: 10.1590/1679-775720130184.
7
Various irrigation protocols for final rinse to improve bond strengths of fiber posts inside the root canal.用于最终冲洗的各种冲洗方案,以提高根管内纤维桩的粘结强度。
Eur J Oral Sci. 2013 Aug;121(4):349-54. doi: 10.1111/eos.12057. Epub 2013 May 18.
8
Effect of cleaning method, luting agent and preparation procedure on the retention of fibre posts.清洗方法、黏结剂和预备程序对纤维桩固位力的影响。
Int Endod J. 2012 Dec;45(12):1116-26. doi: 10.1111/j.1365-2591.2012.02081.x. Epub 2012 Jun 20.
9
Bacterial microleakage and post space timing for two endodontic sealers: an in vitro study.两种根管充填封闭剂的细菌微渗漏及桩道预备时机:一项体外研究
J Mass Dent Soc. 2010 Summer;59(2):34-7.
10
A laboratory comparison of apical leakage between immediate versus delayed post space preparation in root canals filled with Resilon.研究背景:Resilon 根管充填后即刻与延期牙本质预备对根尖微渗漏的影响 目的:比较 Resilon 根管充填后即刻与延期牙本质预备对根尖微渗漏的影响。 方法:选取因正畸需要拔除的前磨牙 60 颗,随机分为 2 组,每组 30 颗。实验组即刻进行牙本质预备,对照组延迟至根管充填后 1 周进行牙本质预备。用 EDTA 凝胶和水门汀将牙本质片黏附在根管内,使用不含麝香草酚的 Fuji II 进行根管充填。将样本浸泡在亚甲蓝溶液中 24 小时后,沿牙体长轴将样本切开,用数码相机拍照,使用 Image J 软件测量蓝色染色的长度。 结果:实验组的平均微渗漏值为(1.11±0.35)mm,对照组的平均微渗漏值为(1.31±0.32)mm,两组间差异有统计学意义(t=2.47,P=0.02)。 结论:Resilon 根管充填后即刻牙本质预备会增加根尖微渗漏,延迟牙本质预备可以减少根尖微渗漏。
Int Endod J. 2010 Sep;43(9):775-81. doi: 10.1111/j.1365-2591.2010.01742.x. Epub 2010 Jul 1.