Penido Alexandre Batista, De Morais Selene Maia, Ribeiro Alan Bezerra, Alves Daniela Ribeiro, Rodrigues Ana Livya Moreira, Dos Santos Leonardo Hunaldo, de Menezes Jane Eire Silva Alencar
Centro de Ciências Sociais Saúde e Tecnologia, Curso de Enfermagem, Universidade Federal do Maranhão, Rua Urbano Santos, s/n, Centro, 65900-410 Imperatriz, MA, Brazil.
Departamento de Química, Laboratório de Química e Produtos Naturais, Universidade Estadual do Ceará, Campus do Itaperi, Av. Dr. Silas Munguba 1.700, 60.714-903 Fortaleza, CE, Brazil.
Evid Based Complement Alternat Med. 2017;2017:1753673. doi: 10.1155/2017/1753673. Epub 2017 Feb 21.
Alzheimer's disease (AD) has been linked with oxidative stress, acetylcholine deficiency in the brain, and inflammatory processes. In the northeast region of Brazil, various plants are used to treat several diseases associated with these processes; then an antioxidant test was performed with those plants in a previous work and twelve species with higher antioxidant activity were selected for AChE inhibition evaluation. The phenolic compounds content was determined by Folin-Ciocalteu test and flavonoid content with AlCl reagent using UV-visible spectrophotometry. The antioxidant activity was assessed analyzing the inhibitory activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonate (ABTS) and by the -carotene/linoleic acid system and acetylcholinesterase inhibition using qualitative and quantitative tests. The combination of better acetylcholinesterase inhibitory and antioxidant activities pointed out six species, in descending order, as the best potential sources of therapeutic agents against AD: . Besides, the phenolic compounds in the species probably contribute to these activities. However, further pharmacological studies to assess the specific applications of these plants against AD are required to confirm these results.
阿尔茨海默病（AD）与氧化应激、大脑中乙酰胆碱缺乏以及炎症过程有关。在巴西东北部，多种植物被用于治疗与这些过程相关的多种疾病；因此，在之前的一项研究中对这些植物进行了抗氧化测试，并选择了12种具有较高抗氧化活性的植物进行乙酰胆碱酯酶抑制评估。通过福林-西奥尔特试剂法测定酚类化合物含量，使用紫外可见分光光度法通过氯化铝试剂测定黄酮类化合物含量。通过分析对2,2-二苯基-1-苦基肼（DPPH）和2,2-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）的抑制活性以及通过β-胡萝卜素/亚油酸体系，并使用定性和定量试验检测乙酰胆碱酯酶抑制作用来评估抗氧化活性。乙酰胆碱酯酶抑制和抗氧化活性的最佳组合指出了六种植物，按降序排列，是对抗AD的最佳潜在治疗剂来源： 。此外，这些植物中的酚类化合物可能有助于这些活性。然而，需要进一步的药理学研究来评估这些植物针对AD的具体应用，以证实这些结果。
