Anti-Inflammatory Activity of the Essential Oil Citral in Experimental Infection with in a Model Air Pouch.

This study proposes to implement an alternative and effective strategy for local treatment of disease provoked by . For the analysis of possible anti-inflammatory activity of essential oil, after establishing an air pouch model, 48 male mice of Balb/c were treated, infected, and euthanized at 4 and 8 h. Thus, the total and differential white blood cells were counted in the animal's blood, and cytokines IL-1, IL-6, and TNF- were titrated using ELISA in the air pouch lavage. Moreover, TNF-, IL-1, and IL-6 gene expression was analyzed through an RT-qPCR array, and was quantified using qPCR. Our results, < 0.05, showed that EOC reduced the quantity of microorganisms. The group of mice treated with essential oil citral showed a significant decrease in TNF- levels in tests demonstrating anti-inflammatory activity. There is no data about the mutual influence of the air pouch model, essential oil citral, and . Thus, considering the interaction of these variables and the anti-inflammatory activity of the essential oil citral, we demonstrated, by alternative local treatment, a new antimicrobial agent that is not an antibiotic.