Quan Nguyen Van, Anh La Hoang, Lam Vu Quang, Takami Akiyoshi, Teschke Rolf, Khanh Tran Dang, Xuan Tran Dang
Transdisciplinary Science and Engineering Program, Graduate School of Advanced Science and Engineering, Hiroshima University, Hiroshima 739-8529, Japan.
Division of Hematology, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute 480-1195, Japan.
Molecules. 2022 Jan 25;27(3):774. doi: 10.3390/molecules27030774.
Essential oils (EOs) of fruits, fruits, and seeds were analyzed for their phytochemical profiles and biological activities, including anti-diabetes, anti-gout, and anti-leukemia properties. Sixty-six volatile compounds were identified by gas chromatography-mass spectrometry (GC-MS), in which, myristicin (68.3%), limonene (44.2%), and linalool (49.3%) were the most prominent components of EOs extracted from , , and , respectively. In addition, only EOs from inhibited the activities of all tested enzymes comprising α-amylase (IC = 7.73 mg/mL), α-glucosidase (IC = 0.84 mg/mL), and xanthine oxidase (IC = 0.88 mg/mL), which are related to type 2 diabetes and gout. Remarkably, all EOs from , (IC = 0.73 mg/mL), and (IC = 1.46 mg/mL) showed a stronger anti-α-glucosidase ability than acarbose (IC = 2.69 mg/mL), a known anti-diabetic agent. Moreover, the growth of leukemia cell Meg-01 was significantly suppressed by all EOs, of which, the IC values were recorded as 0.32, 0.64, and 0.31 mg/mL for EOs from , , and , respectively. As it stands, this is the first report about the inhibitory effects of EOs from and fruits, and seeds on the human leukemia cell line Meg-01 and key enzymes linked to diabetes and gout. In conclusion, the present study suggests that EOs from these natural spices may be promising candidates for pharmaceutical industries to develop nature-based drugs to treat diabetes mellitus or gout, as well as malignant hematological diseases such as leukemia.
对果实、果实和种子的精油进行了植物化学特征和生物活性分析，包括抗糖尿病、抗痛风和抗白血病特性。通过气相色谱 - 质谱联用仪（GC-MS）鉴定出66种挥发性化合物，其中，肉豆蔻醚（68.3%）、柠檬烯（44.2%）和芳樟醇（49.3%）分别是从[具体来源1]、[具体来源2]和[具体来源3]中提取的精油的最主要成分。此外，只有来自[具体来源4]的精油抑制了所有测试酶的活性，这些酶包括α - 淀粉酶（IC50 = 7.73 mg/mL）、α - 葡萄糖苷酶（IC50 = 0.84 mg/mL）和黄嘌呤氧化酶（IC50 = 0.88 mg/mL），它们与2型糖尿病和痛风相关。值得注意的是，来自[具体来源5]、[具体来源6]（IC50 = 0.73 mg/mL）和[具体来源7]（IC50 = 1.46 mg/mL）的所有精油均表现出比阿卡波糖（IC50 = 2.69 mg/mL，一种已知的抗糖尿病药物）更强的抗α - 葡萄糖苷酶能力。此外，所有精油均显著抑制白血病细胞Meg - 01的生长，其中，来自[具体来源8]、[具体来源9]和[具体来源10]的精油的IC50值分别记录为0.32、0.64和0.31 mg/mL。就目前而言，这是关于[具体来源8]和[具体来源9]的果实以及[具体来源10]的种子的精油对人白血病细胞系Meg - 01以及与糖尿病和痛风相关的关键酶的抑制作用的首次报道。总之，本研究表明，这些天然香料的精油可能是制药行业开发基于天然的药物以治疗糖尿病或痛风以及白血病等恶性血液疾病的有前途的候选物。
