Mirzaei A, Sepehri S, Sadeghi H, Alamdari A
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Department of Biotechnology, Fergusson College, Pune - 414001, Maharashtra, India.
J Med Life. 2015;8(Spec Iss 3):96-100.
Pesticides are a critical tool for crop protection and control of different pests and insects. The present research conducted to evaluate the protective role of Jaft extract against oxidative pressure, biochemical variations because of limited time giveaway to carbendazim in Wistar mice males. Fresh fruits of were dried and the internal layer (Jaft) was collected for a hydroalcoholic extract by a maceration method at normal ambient condition. For the experimental study, twenty-four adult male rats (Wistar albino rats weighing 150-200 g) were randomized into 3 teams out of eight. Team I subserved like a vehicle treated group, received corn oil additionally to their food, while the animals in the second team got 0.1 ml carbendazim (50mg/ kg in corn oil) via oral path for nine days. Rats in group III received Jaft (500 mg/ kg orally + in carbendazim for 9 days. Blood samples were obtained by heart puncture to determine alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine, alanine (ALT) and aspartate aminotransferases (AST); by using auto-analyzer in serum.Kidneys and liver separated from rats and provided for series of biochemical parameters homogenization like GSH and MDA stages. The serum content of AST, ALT, ALP, BUN and creatinine were significantly elevated by in carbendazim treatment (group II) compared to the negative group (p<0.01).The liver enzymes operations, creatinine and BUN were significantly reduced in rats (p<0.05) when Jaft was received in a short period of time (group III). Hepatic and GSH and renal MDA stages in group (II) were clearly (p<0.05) enhanced and decreased consequently. The GSH and MDA stages content were significantly normalized in mice (p<0.05) when Jaft was received by group III. According to the present data, Jaft can neutralize carbendazim contain pressure of oxidative and recover the abnormal pathological injuries in Wistar mice males.
农药是作物保护以及防治各类害虫和昆虫的关键工具。本研究旨在评估Jaft提取物对雄性Wistar小鼠因多菌灵导致的氧化应激和生化变化的保护作用。将[具体水果名称]的新鲜果实干燥，收集内层（Jaft），在正常环境条件下通过浸渍法制备水醇提取物。在实验研究中，将24只成年雄性大鼠（体重150 - 200克的Wistar白化大鼠）随机分为8组中的3组。第一组作为溶剂对照组，除食物外还额外给予玉米油，而第二组动物通过口服途径连续九天给予0.1毫升多菌灵（50毫克/千克溶于玉米油）。第三组大鼠口服Jaft（500毫克/千克）并同时给予多菌灵九天。通过心脏穿刺采集血液样本，使用自动分析仪测定血清中的碱性磷酸酶（ALP）、血尿素氮（BUN）、肌酐、丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）。将大鼠的肾脏和肝脏分离，用于检测谷胱甘肽（GSH）和丙二醛（MDA）等一系列生化参数的匀浆。与阴性对照组相比，多菌灵处理组（第二组）的血清AST、ALT、ALP、BUN和肌酐含量显著升高（p<0.01）。当短期内给予Jaft时，大鼠的肝酶活性、肌酐和BUN显著降低（p<0.05）（第三组）。相应地，第二组的肝脏GSH和肾脏MDA水平明显升高和降低（p<0.05）。当第三组给予Jaft时，小鼠的GSH和MDA水平含量显著恢复正常（p<0.05）。根据目前的数据，Jaft可以中和多菌灵引起的氧化压力，并恢复雄性Wistar小鼠的异常病理损伤。
